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首页> 外文期刊>Journal of genetics >Introgression of a leaf rust resistance gene from Aegilops caudata to bread wheat
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Introgression of a leaf rust resistance gene from Aegilops caudata to bread wheat

机译:盾形小麦叶片防锈基因渗入面包小麦

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Rusts are the most important biotic constraints limiting wheat productivity worldwide. Deployment of cultivars with broad spectrum rust resistance is the only environmentally viable option to combat these diseases. Identification and introgression of novel sources of resistance is a continuous process to combat the ever evolving pathogens. The germplasm of nonprogenitor Aegilops species with substantial amount of variability has been exploited to a limited extent. In the present investigation introgression, inheritance and molecular mapping of a leaf rust resistance gene of Ae. caudata (CC) acc. pau3556 in cultivated wheat were undertaken. An F2 population derived from the cross of Triticum aestivum cv.WL711 a€“ Ae. caudata introgression line T291-2 with wheat cultivar PBW343 segregated for a single dominant leaf rust resistance gene at the seedling and adult plant stages. Progeny testing in F3 confirmed the introgression of a single gene for leaf rust resistance. Bulked segregant analysis using polymorphic D-genome-specific SSR markers and the cosegregation of the 5DS anchored markers (Xcfd18, Xcfd78, Xfd81 and Xcfd189) with the rust resistance in the F2 population mapped the leaf rust resistance gene (LrAC) on the short arm of wheat chromosome 5D. Genetic complementation and the linked molecular markers revealed that LrAC is a novel homoeoallele of an orthologue Lr57 already introgressed from the 5M chromosome of Ae. geniculata on 5DS of wheat.
机译:铁锈是限制全球小麦产量的最重要的生物限制因素。部署具有广谱抗锈性的品种是对抗这些疾病的唯一在环境上可行的选择。鉴定和渗入新的抗药性来源是与不断发展的病原体作斗争的一个连续过程。具有很大程度的变异性的非祖先节肢动物物种的种质已被有限地利用。在本研究中,Ae的抗叶锈基因的渗入,遗传和分子作图。 caudata(CC)认证在栽培小麦中进行了pau3556。 F2种群,由普通小麦的杂交种WL711 ae。Ae。杂交而来。带有小麦品种PBW343的caudata渗入系T291-2在苗期和成株期分离出一个单一的优势叶锈病抗性基因。 F3的后代测试证实了单一基因的抗叶锈性基因渗入。使用多态性D基因组特异性SSR标记和5DS锚定标记(Xcfd18,Xcfd78,Xfd81和Xcfd189)与F2群体中的抗锈性的共分离进行的大体积分离分析小麦5D染色体。遗传互补和链接的分子标记表明,LrAC是已经从Ae的5M染色体渗入的直向同源Lr57的新同等位基因。小麦5DS上的Geneticata。

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