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首页> 外文期刊>Journal of Ginseng Research >Effect of Korean Red Ginseng treatment on the gene expression profile of diabetic rat retina
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Effect of Korean Red Ginseng treatment on the gene expression profile of diabetic rat retina

机译：红参对糖尿病大鼠视网膜基因表达谱的影响

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Background Korean Red Ginseng (KRG) is a herbal medicine used in Asian countries and is very popular for its beneficial biological properties. Diabetes mellitus (DM) and its complications are rapidly becoming a global public health concern. The literature on transcriptional changes induced by KRG in rat models of diabetic retinopathy is limited. Considering these facts, we designed this study to determine whether retinopathy-associated genes are altered in retinas of rats with DM and whether the induced changes are reversed by KRG. Methods Male Sprague–Dawley rats were intravenously injected with streptozotocin (50?mg/kg body weight) to induce DM, following which, KRG powder (200?mg/kg body weight) was orally administered to the KRG-treated DM rat group for 10 wks. The rats were then sacrificed, and their retinas were harvested for total RNA extraction. Microarray gene expression profiling was performed on the extracted RNA samples. Results From among > 31,000 genes investigated, the expression of 268 genes was observed to be upregulated and that of 58 genes was downregulated, with twofold altered expression levels in the DM group compared with those in the control group. Moreover, 39 genes were upregulated more than twofold and 84 genes were downregulated in the KRG-treated group compared to the DM group. The expression of the genes was significantly reversed by KRG treatment; some of these genes were analyzed further to verify the results of the microarray experiments. Conclusion Taken together, our data suggest that reversed changes in the gene expression may mediate alleviating activities of KRG in rats with diabetic retinopathy. Keywords: diabetic retinopathy, gene profiling, microarray, Panax ginseng.
机译：背景技术韩国红参（KRG）是一种在亚洲国家/地区使用的草药，并且因其有益的生物学特性而广受欢迎。糖尿病及其并发症正迅速成为全球公共卫生关注的焦点。关于在糖尿病性视网膜病大鼠模型中由KRG诱导的​​转录变化的文献有限。考虑到这些事实，我们设计了这项研究，以确定在患有DM的大鼠视网膜中视网膜病变相关基因是否发生了改变，以及诱导的变化是否被KRG逆转。方法雄性Sprague–Dawley大鼠静脉注射链脲佐菌素（50？mg / kg体重）诱发DM，然后口服KRG散剂（200？mg / kg体重）给予KRG治疗的DM大鼠组， 10周。然后处死大鼠，并收集其视网膜以提取总RNA。对提取的RNA样品进行微阵列基因表达谱分析。结果在研究的31,000多个基因中，观察到268个基因的表达被上调，而58个基因的表达被下调，DM组的表达水平是对照组的两倍。此外，与DM组相比，KRG治疗组的39个基因上调了两倍以上，而84个基因下调了。 KRG处理显着逆转了基因的表达;对其中的一些基因进行了进一步分析，以验证微阵列实验的结果。结论综上所述，我们的数据表明，基因表达的反向改变可能介导了糖尿病视网膜病变大鼠KRG的缓解作用。关键词：糖尿病视网膜病变，基因谱分析，芯片，人参。

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