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首页> 外文期刊>Journal of genetics >Development of molecular map and identification of QTLs linked to Fusarium wilt resistance in chickpea
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Development of molecular map and identification of QTLs linked to Fusarium wilt resistance in chickpea

机译:鹰嘴豆枯萎病抗性相关分子图谱的建立和QTLs的鉴定

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A number of genetic maps for Fusarium wilt resistance in chickpea have been reported in earlier studies, however QTLs identified for Fusarium wilt resistance were unstable. Hence, the present study aims to map novel molecular markers and to identify QTLs for Fusarium wilt resistance in chickpea. An intraspecific linkage map of chickpea (Cicer arietinum L.) was constructed using F10a€“F11 recombinant inbred lines (RILs) derived from a cross between K850 and WR315 segregating for H2 locus. A set of 31 polymorphic simple sequence repeat (SSR) markers obtained by screening 300 SSRs and were used for genotyping. The linkage map had four linkage groups and coverage of 690 cM with a marker density of 5.72 cM. The RILs were screened for their wilt reaction across two seasons in wilt sick plot at International Crop Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India. Five major quantitative trait loci (QTLs) were detected in both seasons for late wilting (60 days after sowing). A stable QTL (GSSR 18-TC14801) for wilt resistance was identified in both the seasons, and the QTL explained a variance of 69.80 and 60.80% in 2007 and 2008 rabi respectively.
机译:在较早的研究中已经报道了许多鹰嘴豆枯萎病抗性的遗传图谱,但是鉴定出的枯萎病抗性的QTL不稳定。因此,本研究旨在绘制新型分子标记,并鉴定鹰嘴豆对枯萎病抗性的QTL。利用F10a–F11重组自交系(RIL)构建了鹰嘴豆(Cicer arietinum L.)的种内连锁图谱,该重组自交系来自K850和WR315之间的杂交,分离出H2基因座。通过筛选300个SSR获得了一组31个多态性简单序列重复(SSR)标记,并用于基因分型。连锁图具有四个连锁组,覆盖范围为690 cM,标记密度为5.72 cM。在印度海得拉巴国际半干旱热带作物研究所(ICRISAT),在两个季节的RIL中筛选了RIL的枯萎反应。在两个季节中均检测到五个主要的数量性状基因座(QTL),用于后期萎ting(播种后60天)。在两个季节中均确定了稳定的抗青枯病QTL(GSSR 18-TC14801),并且QTL解释了2007年和2008年狂犬病的变异分别为69.80%和60.80%。

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