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首页> 外文期刊>Journal of food and drug analysis >Optimization conditions for extracting polysaccharide from Angelica sinensis and its?antioxidant activities
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Optimization conditions for extracting polysaccharide from Angelica sinensis and its?antioxidant activities

机译：当归多糖的提取工艺优化及抗氧化活性

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In this study, polysaccharides from Angelica sinensis were extracted using the ultrasound-assisted extraction method. Based on the results of single factor experiments and orthogonal tests, three independent variables—water/raw material ratio, ultrasound time, and ultrasound power—were selected for investigation. Then, we used response surface methodology to optimize the extraction conditions. The experimental data were fitted to a quadratic equation using multiple regression analysis, and the optimal conditions were as follows: water/raw material ratio, 43.31?mL/g; ultrasonic time, 28.06 minutes; power, 396.83?W. Under such conditions, the polysaccharide yield was 21.89 ± 0.21%, which was well matched with the predicted yield. In?vitro assays, scavenging activity of superoxide anion radicals, hydroxyl radicals, and 2,2-diphenyl-1-picry-hydrazyl radical showed that polysaccharides had certain antioxidant activities and that hydroxyl radicals have a remarkable scavenging capability. Therefore, these studies provide reference for further research and rational development of A. sinensis polysaccharide.
机译：本研究采用超声波辅助提取方法提取当归多糖。根据单因素实验和正交试验的结果，选择了三个独立变量-水/原料比，超声时间和超声功率-进行研究。然后，我们使用响应面方法优化了提取条件。使用多元回归分析将实验数据拟合为二次方程，最佳条件如下：水/原料比为43.31？mL / g;水/原料比为43.31mL / g;超声波时间28.06分钟;功率396.83？W。在这种条件下，多糖得率为21.89±0.21％，与预测的得率非常匹配。在体外试验中，超氧化物阴离子自由基，羟基自由基和2,2-二苯基-1-吡喃肼基自由基的清除活性表明多糖具有一定的抗氧化活性，而羟基自由基具有明显的清除能力。因此，这些研究为进一步研究和合理开发中华曲霉多糖提供了参考。

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