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首页> 外文期刊>Journal of Ginseng Research >EST-SSR Marker Sets for Practical Authentication of All Nine Registered Ginseng Cultivars in Korea
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EST-SSR Marker Sets for Practical Authentication of All Nine Registered Ginseng Cultivars in Korea

机译：EST-SSR标记集，用于韩国所有九个注册人参品种的实际认证

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Panax ginseng has been cultivated for centuries, and nine commercial cultivars have been registered in Korea. However, these nine elite cultivars are grown in less than 10% of ginseng fields, and there is no clear authentication system for each cultivar even though their values are higher than those of local landraces. Here, we have developed 19 microsatellite markers using expressed gene sequences and established an authentication system for all nine cultivars. Five cultivars, ‘Chunpoong’, ‘Sunpoong’, ‘Gumpoong’, ‘Sunun’, and ‘Sunone’, can each be identified by one cultivar-unique allele, gm47n-a, gm47n-c, gm104-a, gm184-a (or gm129-a), and gm175-c, respectively. ‘Yunpoong’ can be identified by the co-appearance of gm47n-b and gm129-c. ‘Sunhyang’ can be distinguished from the other eight cultivars by the co-appearance of gm47n-b, gm129-b, and gm175-a. The two other cultivars, ‘Gopoong’ and ‘Cheongsun’, can be identified by their specific combinations of five marker alleles. This marker set was successfully utilized to identify the cultivars among 70 ginseng individuals and to select true F1 hybrid plants between two cultivars. We further analyzed the homogeneity of each cultivar and phylogenetic relationships among cultivars using these markers. This marker system will be useful to the seed industry and for breeding of ginseng. Keywords: Panax ginseng , Seed industry, Breeding, EST-SSR markers, Cultivar authentication.
机译：人参已经栽培了几个世纪，在韩国已经注册了9个商业品种。但是，这9个优良品种的人均耕种量不到10％，即使每个品种的价值都高于当地品种，也没有明确的认证体系。在这里，我们使用表达的基因序列开发了19个微卫星标记，并为所有9个品种建立了认证系统。可以通过一个品种独特的等位基因gm47n-a，gm47n-c，gm104-a，gm184-a来识别5个品种'Chunpoong'，'Sunpoong'，'Gumpoong'，'Sunun'和'Sunone' （或gm129-a）和gm175-c。可以通过gm47n-b和gm129-c的共同出现来识别“ Yunpoong”。 gm47n-b，gm129-b和gm175-a的共同出现可以将“ Sunhyang”与其他八个品种区分开。可以通过五个标记等位基因的特定组合来识别其他两个品种'Gopoong'和'Cheongsun'。此标记集已成功用于在70个人参个体中鉴定品种，并在两个品种之间选择真正的F1杂种植物。我们使用这些标记进一步分析了每个品种的同质性和品种之间的系统发生关系。该标记系统将对种子产业和人参育种有用。关键词：人参，种子产业，育种，EST-SSR标记，品种认证。

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