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首页> 外文期刊>Journal of Ginseng Research >Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector
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Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector

机译：超高效液相色谱-飞行时间串联质谱-高效液相色谱-紫外检测器对三七叶中皂苷的定性和定量分析

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Background Panax notoginseng leaves (PNL) exhibit extensive activities, but few analytical methods have been established to exclusively determine the dammarane triterpene saponins in PNL. Methods Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF MS) and HPLC-UV methods were developed for the qualitative and quantitative analysis of ginsenosides in PNL, respectively. Results Extraction conditions, including solvents and extraction methods, were optimized, which showed that ginsenosides Rc and Rb3, the main components of PNL, are transformed to notoginsenosides Fe and Fd, respectively, in the presence of water, by removing a glucose residue from position C-3 via possible enzymatic hydrolysis. A total of 57 saponins were identified in the methanolic extract of PNL by UPLC/Q-TOF MS. Among them, 19 components were unambiguously characterized by their reference substances. Additionally, seven saponins of PNL—ginsenosides Rb1, Rc, Rb2, and Rb3, and notoginsenosides Fc, Fe, and Fd—were quantified using the HPLC-UV method after extraction with methanol. The separation of analytes, particularly the separation of notoginsenoside Fc and ginsenoside Rc, was achieved on a Zorbax ODS C8 column at a temperature of 35°C. This developed HPLC-UV method provides an adequate linearity ( r 2 > 0.999), repeatability (relative standard deviation, RSD Conclusion These findings are beneficial to the quality control of PNL and its relevant products.
机译：背景三七叶（PNL）表现出广泛的活性，但建立专门用于确定PNL中的三烯三萜皂苷的分析方法很少。方法建立了超高效液相色谱-飞行时间质谱（UPLC / Q-TOF MS）和HPLC-UV方法，分别对PNL中人参皂苷进行定性和定量分析。结果优化了提取条件，包括溶剂和提取方法，结果表明，PNL的主要成分人参皂苷Rc和Rb3在存在水的情况下，通过从位置去除葡萄糖残基，分别转化为三七皂苷Fe和Fd。 C-3通过可能的酶水解。通过UPLC / Q-TOF MS在PNL的甲醇提取物中共鉴定出57种皂苷。其中，有19种成分的参考物质明确标明。此外，在用甲醇萃取后，使用HPLC-UV方法对七种PNL皂苷（人参皂甙Rb1，Rc，Rb2和Rb3以及三七皂甙Fc，Fe和Fd）进行了定量。在Zorbax ODS C8色谱柱上于35°C的温度下完成分析物的分离，特别是三七皂苷Fc和人参皂苷Rc的分离。这种发达的HPLC-UV方法可提供足够的线性（r 2

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