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首页> 外文期刊>Journal of Ginseng Research >Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth
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Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth

机译：黑参激活Akt信号传导，从而增强成肌细胞分化和肌管生长

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Background Black ginseng (BG) has greatly enhanced pharmacological activities relative to white or red ginseng. However, the effect and molecular mechanism of BG on muscle growth has not yet been examined. In this study, we investigated whether BG could regulate myoblast differentiation and myotube hypertrophy. Methods BG-treated C2C12 myoblasts were differentiated, followed by immunoblotting for myogenic regulators, immunostaining for a muscle marker, myosin heavy chain or immunoprecipitation analysis for myogenic transcription factors. Results BG treatment of C2C12 cells resulted in the activation of Akt, thereby enhancing heterodimerization of MyoD and E proteins, which in turn promoted muscle-specific gene expression and myoblast differentiation. BG-treated myoblasts formed larger multinucleated myotubes with increased diameter and thickness, accompanied by enhanced Akt/mTOR/p70S6K activation. Furthermore, the BG treatment of human rhabdomyosarcoma cells restored myogenic differentiation. Conclusion BG enhances myoblast differentiation and myotube hypertrophy by activating Akt/mTOR/p70S6k axis. Thus, our study demonstrates that BG has promising potential to treat or prevent muscle loss related to aging or other pathological conditions, such as diabetes.
机译：背景黑参（BG）相对于白参或红参具有很大的药理活性。但是，尚未研究BG对肌肉生长的影响和分子机制。在这项研究中，我们调查了BG是否可以调节成肌细胞分化和肌管肥大。方法区分经BG处理的C2C12成肌细胞，然后进行免疫印迹法检测成肌调节剂，对肌标记物进行免疫染色，对肌球蛋白重链进行免疫沉淀或对成肌转录因子进行免疫沉淀分析。结果BG处理C2C12细胞可激活Akt，从而增强MyoD和E蛋白的异源二聚化，进而促进肌肉特异性基因表达和成肌细胞分化。 BG处理的成肌细胞形成较大的多核肌管，直径和厚度增加，并伴有增强的Akt / mTOR / p70S6K激活。此外，对人横纹肌肉瘤细胞的BG治疗恢复了肌原性分化。结论BG通过激活Akt / mTOR / p70S6k轴增强成肌细胞分化和肌管肥大。因此，我们的研究表明，BG具有治疗或预防与衰老或其他病理状况（例如糖尿病）相关的肌肉丢失的潜力。

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