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首页> 外文期刊>Journal of Ginseng Research >Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice
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Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice

机译：人参皂甙Rg1增强小鼠骨骼肌的氧化代谢和合成代谢反应

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Background The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in?vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5?weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in?vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.
机译：背景人参皂苷Rg1已显示出多种有益健康的药理活性。以前，我们已经报道Rg1促进C2C12成肌细胞中的肌源性分化和肌管生长。在这项研究中，研究了Rg1对骨骼肌纤维类型组成和氧化代谢的体内作用。方法为研究Rg1对骨骼肌的作用，对3个月大的小鼠用Rg1治疗5周。为了评估肌肉强度，进行了握力测试，并收集了下后肢的肌肉，然后进行了各种详细的分析，例如组织学染色，免疫印迹，免疫染色和实时定量逆转录聚合酶链反应。此外，为了验证体内数据，用Rg1处理从小鼠分离的原代成肌细胞，并通过免疫印迹和免疫染色分析检查Rg1对肌管生长的影响。结果Rg1处理增加了具有氧化性和糖酵解性肌纤维的肌球蛋白重链同工型的表达。肌纤维大小增加，同时肌肉力量增强。 Rg1治疗还通过增加氧化磷酸化蛋白来增强氧化肌代谢。此外，Rg1处理的肌肉表现出增加的合成代谢S6激酶信号传导水平。结论Rg1通过增强小鼠的肌肉基因表达和氧化性肌肉代谢来改善肌肉功能。

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