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首页> 外文期刊>Journal of Ginseng Research >Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I
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Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I

机译：多人参茎叶人参皂苷与I型人参糖苷酶反应的动态变化

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Background Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from Aspergillus niger g.848. Methods NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR. Results All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred through Rb3→C-Mx1→C-Mx; when reacted for 1?h, Rb3 decreased from 27.1% to 9.82 %, C-Mx1 increased from 15.5% to 32.3%, C-Mx was produced to 6.46%, finally into C-Mx and a small amount of C-K. When reacted for 1.5?h, all the Rb1, Rd, and Gyp17 were completely reacted, and the reaction intermediate F2 was produced to 8.25%, finally into C-K. The main reaction of Rc (13.8%) occurred through Rc→C-Mc1→C-Mc→C-K. The enzyme barely hydrolyzed the terminal xyloside on 3- O — or 20- O -sugar-moiety of the substrate; therefore, 9.43?g C-Mx, 6.85?g C-K, 4.50?g R7, and 4.71?g Fc (hardly separating from the substrate) were obtained from 50?g NGL ginsenosides by the crude enzyme reaction. Conclusion Four monomer ginsenosides were successfully produced and separated from NGL ginsenosides by the enzyme reaction.
机译：背景三七茎叶（NGL）人参皂苷尚未得到很好的利用。为了提高其利用率，使用来自黑曲霉g.848的I型人参糖苷酶研究了NGL人参皂苷的生物转化。方法在RAT-5D生物反应器中使NGL人参皂甙与粗酶发生反应，通过HPLC鉴定NGL中人参皂甙的动态变化。使用硅胶柱分离反应产物，并通过HPLC和NMR鉴定。结果所有的NGL人参皂苷均为原人参二醇型人参皂苷。人参皂苷的主要含量为27.1％Rb3、15.7％C-Mx1、13.8％Rc，11.1％Fc，7.10％Fa，6.44％C-Mc，5.08％Rb2和4.31％Rb1。在NGL人参皂苷与粗酶的反应中，Rb3与C-Mx1的主要反应是通过Rb3→C-Mx1→C-Mx发生的。当反应1？h时，Rb3从27.1％降低到9.82％，C-Mx1从15.5％增加到32.3％，C-Mx产生到6.46％，最后变成C-Mx和少量C-K。当反应1.5小时时，所有的Rb1，Rd和Gyp17完全反应，反应中间体F2的产率为8.25％，最后变成C-K。 Rc的主要反应（13.8％）通过Rc→C-Mc1→C-Mc→C-K发生。该酶几乎不水解底物的3- O-或20-O-糖基上的末端木糖苷;因此，通过粗酶反应从50微克NGL人参皂苷中获得了9.43微克C-Mx，6.85微克C-K，4.50微克R7和4.71微克Fc（几乎不与底物分离）。结论成功地生产了四种人参皂苷，并通过酶反应将其与NGL人参皂苷分离。

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