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首页> 外文期刊>Journal of Experimental Neuroscience >Local cell proliferation upon enucleation in Direct Retinal Brain Targets in the Visual system of the Adult Mouse:
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Local cell proliferation upon enucleation in Direct Retinal Brain Targets in the Visual system of the Adult Mouse:

机译：成年小鼠视觉系统中直接视网膜脑目标摘除后局部细胞增殖：

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In this study we used incorporation of the DNA synthesis marker 5-bromo-2′-deoxyuridine or BrdU to visualize cell proliferation in the visual system of the adult mouse as a response to monocular enucleation. We detected new BrdU-labeled cells in different subcortical retinal target regions and we established a specific time frame in which this cell proliferation occurred. By performing immunofluorescent double stainings for BrdU and different vascular (glucose transporter type 1, collagen type IV), glial (thymosin β4, glial fibrillary acidic protein) and neuronal (Neuronal Nuclei, doublecortin) markers, we identified these proliferating cells as activated microglia. Additional immunohistochemical stainings for thymosin β4 and glial fibrillary acidic protein also revealed reactive astrocytes in the different retinorecipient nuclei and allowed us to delineate a time frame for microglial and astroglial activation. A PCR array experiment further showed increased levels of cytokines, chemokines, growth factors and enzymes that play an important role in microglial-astroglial communication during the glial activation process in response to the deafferentation.
机译：在这项研究中，我们使用了DNA合成标记物5-bromo-2'-deoxyuridine或BrdU的结合，以观察成年小鼠视觉系统中的细胞增生，作为对单眼摘除的反应。我们在不同的皮质下视网膜靶区域中检测到新的BrdU标记的细胞，并建立了发生这种细胞增殖的特定时间范围。通过对BrdU和不同血管（葡萄糖转运蛋白1型，IV型胶原蛋白），神经胶质（胸腺素β4，神经胶质纤维酸性蛋白）和神经元（神经元核，双皮质素）标记物进行免疫荧光双重染色，我们将这些增殖细胞鉴定为活化的小胶质细胞。胸腺素β4和神经胶质原纤维酸性蛋白的其他免疫组化染色也显示了不同的视网膜受体核中的反应性星形胶质细胞，这使我们能够划定小胶质细胞和星形胶质细胞活化的时间范围。 PCR阵列实验进一步显示，细胞质因子，趋化因子，生长因子和酶的水平升高，这些水平在神经胶质细胞激活过程中对脱除咖啡因引起的小胶质-星形胶质细胞通讯中起重要作用。

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