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首页> 外文期刊>Journal of Health Disparities Research and Practice >Survey of Virus Diseases of Taro, Colocasia esculenta, on the Aunu’u Island of American Samoa
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Survey of Virus Diseases of Taro, Colocasia esculenta, on the Aunu’u Island of American Samoa

机译：美属萨摩亚Aunu’u岛芋芋芋头病毒病调查

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Taro, Colocasia esculenta, is the most important staple crop in American Samoa, especially in Aunu’u, a small (1.5 km2) island, approximately 2 km southeast of the main island of Tutuila. However, the sustainability of taro production is threatened by the occurrence of numerous diseases and pests, as evidenced by the leaf blight (Phytophthora colocasiae) epidemic that devastated the Samoan taro production in early 1990s. Crop losses due to viruses alone have been estimated at 20 - 60%. The objective of this study was to investigate the incidence of virus diseases of taro in Aunu’u. Overall, 112 leaf samples, mostly (85%) from cultivars “Talo Manua” and “Palau 10”, were collected from seven major farms on the Island. Genomic DNA and total RNA were extracted from each sample, and the extracts subjected to PCR and RT-PCR, using primers that amplify specific regions of the target virus genome. The reactions were resolved on 1.5% agarose gel electrophoresis, and gels were analyzed using a GelDoc EZ documentation system. Dasheen Mosaic Virus (DsMV) was the predominant virus in Aunu’u, infecting 79% of plants, followed by Taro Bacilliform Virus (TaBV), with 46%. Many (40%) of the plants infected with DsMV were co-infected with TaBV. Cultivar “Talo Manua” was more susceptible to both viruses than “Palau 10”. No other taro virus was detected. This is the first report on taro virus diseases in Aunu’u. It would be desirable to extend this type of survey to other islands of American Samoa.
机译：芋头芋，是美属萨摩亚最重要的主粮，尤其是在图图伊拉主岛东南约2公里的小岛（1.5平方公里）奥努阿。但是，芋头生产的可持续性受到多种疾病和害虫的威胁，1990年代初叶枯萎病（Phytophthora colocasiae）流行证明了萨摩亚芋头生产受到破坏。仅由于病毒造成的作物损失估计为20-60％。这项研究的目的是调查Aunu’u芋头病毒病的发病率。总体上，从岛上的七个主要农场收集了112个叶样品，其中大部分（85％）来自“ Talo Manua”和“ Palau 10”品种。从每个样品中提取基因组DNA和总RNA，并使用扩增靶病毒基因组特定区域的引物对提取物进行PCR和RT-PCR。将反应在1.5％琼脂糖凝胶电泳上分离，并使用GelDoc EZ文件系统分析凝胶。 Dasheen花叶病毒（DsMV）是Aunu’u的主要病毒，感染了79％的植物，其次是Taro Bacilliform病毒（TaBV），感染了46％。用DsMV感染的植物中有许多（40％）被TaBV共同感染。品种“ Talo Manua”比“ Palau 10”更容易受到两种病毒的侵害。没有检测到其他芋头病毒。这是奥努阿关于芋头病毒病的第一份报告。希望将这类调查扩展到美属萨摩亚的其他岛屿。

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