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首页> 外文期刊>Journal of experimental orthopaedics. >Optimization of leukocyte-poor platelet-rich plasma preparation: a validation study of leukocyte-poor platelet-rich plasma obtained using different preparer, storage, and activation methods
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Optimization of leukocyte-poor platelet-rich plasma preparation: a validation study of leukocyte-poor platelet-rich plasma obtained using different preparer, storage, and activation methods

机译：优化贫白细胞贫血小板血浆的制备方法：使用不同的制备，保存和激活方法获得的贫白细胞贫血小板血浆的验证研究

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Abstract BackgroundAlternative methods of platelet-rich plasma (PRP) preparation, storage, and activation that can be stably reproduced are needed to improve PRP production. The purpose of this study was to investigate the effect of the preparer’s experience on the quality of prepared PRP, chronological changes occurring in PRP, and the effect of the activation procedures on the release of several growth factors from PRP, using PRP prepared with the PRGF-Endoret Kit.MethodsLeukocyte-poor PRP samples from seventeen healthy volunteers were prepared using the PRGF-Endoret Kit and the PRGF IV System Centrifuge. The platelet and leukocyte concentrations were compared based on the preparer’s experience. The concentrations of platelets, hepatocyte growth factor (HGF), platelet-derived growth factor-BB (PDGF-BB), and insulin-like growth factor-1 (IGF-1) were determined at 0 and 60?min after PRP preparation, and compared. Concentrations of the above growth factors from PRP activated by freeze–thaw cycling and by calcium chloride (CaCl2) were also compared.ResultsNo significant difference was observed in the platelet concentrations and leukocyte contamination rates, based on the preparer’s experience. At 60?min after PRP preparation, the platelet concentration decreased significantly, while the HGF, PDGF-BB, and IGF-1 concentrations remained unchanged. Activation with CaCl2 resulted in a significant increase in the PDGF-BB levels, although the HGF and IGF-1 concentrations remained unchanged.ConclusionsThe results of this study show that leukocyte-poor PRP prepared using the PRGF-Endoret Kit did not result in any qualitative difference that depended on the experience of the preparer. However, PRP preparation required standardization in terms of the time of blood count measurement. Growth factor concentrations in PRP differed according to the platelet-activation method used.
机译：摘要背景需要稳定地复制富血小板血浆（PRP）的制备，储存和活化的替代方法，以提高PRP的产量。这项研究的目的是研究使用PRGF制备的PRP，研究准备者的经验对制备的PRP的质量，PRP中发生的时间变化以及激活程序对从PRP释放多种生长因子的影响。方法使用PRGF-Endoret试剂盒和PRGF IV系统离心机制备了17名健康志愿者的白细胞贫乏PRP样品。根据制备者的经验比较血小板和白细胞的浓度。在制备PRP后0和60？min分别测定血小板，肝细胞生长因子（HGF），血小板衍生生长因子BB（PDGF-BB）和胰岛素样生长因子-1（IGF-1）的浓度，并进行比较。还比较了冻融循环和氯化钙（CaCl2）激活的PRP中上述生长因子的浓度。结果，根据制备者的经验，血小板浓度和白细胞污染率没有显着差异。制备PRP后60分钟，血小板浓度显着下降，而HGF，PDGF-BB和IGF-1浓度保持不变。尽管HGF和IGF-1浓度保持不变，但用CaCl2激活导致PDGF-BB水平显着增加。结论本研究结果表明，使用PRGF-Endoret试剂盒制备的贫白细胞PRP不会产生任何定性差异取决于准备者的经验。但是，PRP的制备需要根据血球计数时间进行标准化。 PRP中的生长因子浓度根据所使用的血小板激活方法而有所不同。

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