In this study,the gelatin gum arabic m icrocapsule s loaded B acillus thuringiensis Bt wereprepared by complex coacervation . Based on single factor experiment and o rthogonal tes t,the preparation process parameters such as encapsulating temperature and pH, theconcentration of getatin and gum Arabic , and rotational speed were investigated andoptimi z ed . The obtained optimum parameters of preparation were as follow s: theencapsulating temperature and pH were 4 0 ℃ and 3.9 the concentration of getatin and gumarabic were 10 g 1 L 1 , the volume rati o of core 1010 cfu/L Bt suspension) to wall 10 g 1 L 1gelatin and Arabia gum solution was 1:2, and stirring speed was 350 rpm. With theoptimized preparation process t he size and span value of obtained Bt microcapsule w ereabout 12.7 μm and 1.03, and encapsulation e fficiency was up to 87%87%. The experiment ofbiological activity to grub showed that the Bt microcapsule had better persistent eff ectc ompared with the control , w hen the number of spores was 109cfu 1 ml 1 , the mortality ofgrub w ere 75.65% and 86.09% on the 7th and the 14th day .
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机译：本研究采用复合凝聚法制备了载有苏云金芽孢杆菌Bt的明胶阿拉伯胶微胶囊。在单因素试验和正交试验的基础上，研究并优化了包封温度，pH值，阿拉伯明胶和阿拉伯树胶的浓度，转速等制备工艺参数。得到的最佳制备参数为：包封温度和pH为4 0℃，3.9的角质和阿拉伯胶的浓度为10 g 1 L 1，核心1010 cfu / L Bt悬浮液的体积比为10。 g 1 L 1明胶和阿拉伯树胶溶液的比例为1：2，搅拌速度为350 rpm。通过优化的制备工艺，获得的Bt微囊的大小和跨度值分别约为12.7μm和1.03，包封效率高达87％87％。对rub的生物活性实验表明，与对照相比，Bt微囊具有更好的持久性，当孢子数为109cfu 1 ml 1时，在第7和14天rub的死亡率分别为75.65％和86.09％。 。
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