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首页> 外文期刊>Journal of immunology research. >Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients
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Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients

机译：固体器官与干细胞移植受者循环中的可变EBV DNA负载分布和异质EBV mRNA表达模式

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摘要

Epstein-Barr virus (EBV) driven post-transplant lymphoproliferative disease (PTLD) is a heterogeneous and potentially life-threatening condition. Early identification of aberrant EBV activity may prevent progression to B-cell lymphoma. We measured EBV DNA load and RNA profiles in plasma and cellular blood compartments of stem cell transplant (SCT; ), solid organ transplant recipients (SOT; ), and SOT having chronic elevated EBV-DNA load . In SCT, EBV DNA was heterogeneously distributed, either in plasma or leukocytes or both. In SOT, EBV DNA load was always cell associated, predominantly in B cells, but occasionally in T cells (CD4 and CD8) or monocytes. All SCT with cell-associated EBV DNA showed BARTs and EBNA1 expression, while LMP1 and LMP2 mRNA was found in 1 and 3 cases, respectively. In SOT, expression of BARTs was detected in all leukocyte samples. LMP2 and EBNA1 mRNA was found in 5/15 and 2/15, respectively, but LMP1 mRNA in only 1, coinciding with severe PTLD and high EBV DNA. Conclusion EBV DNA is differently distributed between white cells and plasma in SOT versus SCT. EBV RNA profiling in blood is feasible and may have added value for understanding pathogenic virus activity in patients with elevated EBV-DNA.
机译：由爱泼斯坦巴尔病毒（EBV）引起的移植后淋巴组织增生性疾病（PTLD）是一种异质性疾病，可能危及生命。早期识别异常的EBV活性可能会预防发展为B细胞淋巴瘤。我们测量了干细胞移植（SCT;），实体器官移植受者（SOT;）和患有EBV-DNA慢性负荷的SOT的血浆和细胞血室中的EBV DNA负荷和RNA谱。在SCT中，EBV DNA异质分布在血浆或白细胞或两者中。在SOT中，EBV DNA负载总是与细胞相关，主要是在B细胞中，但偶尔在T细胞（CD4和CD8）或单核细胞中。所有带有细胞相关EBV DNA的SCT均显示BARTs和EBNA1表达，而分别在1例和3例中发现了LMP1和LMP2 mRNA。在SOT中，在所有白细胞样品中均检测到BARTs的表达。 LMP2和EBNA1 mRNA分别在5/15和2/15中发现，而LMP1 mRNA仅在1/15中发现，与严重的PTLD和高的EBV DNA吻合。结论在SOT和SCT中，EBV DNA在白细胞和血浆之间的分布不同。在血液中进行EBV RNA谱分析是可行的，对于了解EBV-DNA升高的患者的病原性病毒活性可能具有附加价值。

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