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Microbial Community from MTBE-Contaminated Soil for Aerobic Biodegradation of MTBE

机译:来自MTBE污染土壤的微生物群落对MTBE的需氧生物降解

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This Methyl tert-butyl ether (MTBE) is one of the main additives in gasoline toincrease octane rating and consequently reduce air pollution. Thephysico-chemical properties of this substance (high water solubility, lowsorption in soil) result in high mobility and considerable concentrations inaquifers. In this survey, Isfehan Refinery that was encountered with MTBEcontamination problem was selected as a case study and the MTBE degradationability of this contaminated area by its indigenous microorganisms wasinvestigated. In the first step of this survey, the influence of variousfactors on the aerobic degradation of MTBE such as mixed culture type,incubation time, microbial culture and optimal concentration of MTBE wereinvestigated in shaking flasks and the most important factors were specified bymeans of fractional factorial design 1/2. In the second stage by using optimalvalues which obtained from the first stage, the effects of co-substareparameter and inoculum parameter were assayed by means of response surfacemethod. The results of the experiments showed that the mixed culture type andinitial concentration of MTBE were the most significant factors. The results ofthe experiments showed that the mixed indigenous culture acted better thanactivated sludge. The initial concentration of MTBE was also one of the mostsignificant factors. At the best condition about 31 percent of MTBE was treatedby co-substrating with n-hexane in a ratio of 0.2.
机译:这种甲基叔丁基醚(MTBE)是汽油中主要的添加剂之一,可以提高辛烷值,从而减少空气污染。该物质的物理化学性质(高水溶性,在土壤中的低吸收)导致高迁移率和相当大的含水层浓度。在本次调查中,选择了遇到MTBE污染问题的Isfehan精炼厂作为案例研究,并研究了该污染区的本地微生物对其MTBE的降解能力。在这项调查的第一步中,研究了摇瓶中混合培养类型,孵育时间,微生物培养和最佳MTBE浓度等因素对MTBE好氧降解的影响,并通过分数阶乘设计的方法确定了最重要的因素。 1/2。在第二阶段中,通过使用从第一阶段获得的最佳值,通过响应表面方法来分析共亚星参数和接种参数的影响。实验结果表明,MTBE的混合培养类型和初始浓度是最重要的因素。实验结果表明,混合土著文化的表现优于活性污泥。 MTBE的初始浓度也是最重要的因素之一。在最佳条件下,通过与正己烷以0.2的比例共取代来处理约31%的MTBE。

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