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首页> 外文期刊>Journal of innovative optical health sciences >Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications
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Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications

机译：用于FLIM和STED成像应用的绿色发射的CdSe @ ZnS量子点

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Inorganic quantum dots (QDs) have excellent optical properties, such as high fluorescence intensity, excellent photostability and tunable emission wavelength, etc., facilitating them to be used as labels and probes for bioimaging. In this study, [email protected] QDs are used as probes for Fluorescence lifetime imaging microscope (FLIM) and stimulated emission depletion (STED) nanoscopy imaging. The emission peak of [email protected] QDs centered at 526nm with a narrow width of 19nm and the photoluminescence quantum yield (PLQY) was 64%. The QDs presented excellent anti-photobleaching property which can be irradiated for 400min by STED laser with 39.8mW.?The lateral resolution of 42.0nm is demonstrated for single QDs under STED laser (27.5mW) irradiation. Furthermore, the [email protected] QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD probes. Meanwhile, Eca-109 cells labeled with the [email protected] QDs was observed with FLIM, and their fluorescence lifetime was around 3.1ns, consistent with the in vitro value, suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.
机译：无机量子点（QD）具有出色的光学特性，例如高荧光强度，出色的光稳定性和可调的发射波长等，从而使其可以用作生物成像的标记和探针。在这项研究中，[受电子邮件保护的] QD用作荧光寿命成像显微镜（FLIM）和受激发射损耗（STED）纳米显微镜成像的探针。受[电子邮件保护]的量子点的发射峰集中在526nm，窄宽度为19nm，光致发光量子产率（PLQY）为64％。这些量子点具有出色的抗光漂白性能，可以用39.8mW的STED激光照射400分钟。？在27.5mW的STED激光下，单量子点的横向分辨率为42.0nm。此外，[电子邮件保护的] QD首次用于成功标记活HeLa细胞的溶酶体，并且获得了81.5nm的横向分辨率，表明在无机QD探针的活细胞中可利用的超分辨率应用。同时，用FLIM观察到带有[电子邮件保护] QD标记的Eca-109细胞，其荧光寿命约为3.1ns，与体外值一致，这表明QD可以作为进一步FLIM-STED的令人满意的探针实验。

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