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首页> 外文期刊>Journal of International Medical Research >High-dose Propofol Triggers Short-term Neuroprotection and Long-term Neurodegeneration in Primary Neuronal Cultures from Rat Embryos
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High-dose Propofol Triggers Short-term Neuroprotection and Long-term Neurodegeneration in Primary Neuronal Cultures from Rat Embryos

机译：大剂量的异丙酚触发大鼠胚胎原代神经元培养物中的短期神经保护和长期神经退行性变

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This study investigated the effects of propofol on primary neuronal cultures from rat embryos. Primary cortical neuronal cultures were prepared from Wistar rat embryos (E18). The viability of cells exposed to 0.01, 0.1 or 1 mg/ml propofol for up to 48 h was assessed using a methyltetrazolium assay. In order to evaluate the role of γ-aminobutyric acid-A (GABAA) receptors, cells were also pre-incubated with the GABAA-receptor antagonists, gabazine and picrotoxin. Propofol at a concentration of 1 mg/ml significantly reduced cell viability after 12 h. In contrast, this concentration led to a significant increase in cell viability at 3 and 6 h. The GABAA-receptor antagonists did not influence the neurodegenerative effect of propofol but abolished its neuroprotective effect. DNA fragmentation as a marker of apoptosis was elevated after 24 h propofol treatment. These results confirm that high doses of propofol can cause GABAA-receptor triggered neuroprotection and a subsequent time-dependent, but GABAA-independent, neurodegeneration in primary cortical neurons.
机译：这项研究调查了异丙酚对大鼠胚胎原代神经元培养的影响。从Wistar大鼠胚胎（E18）制备初级皮层神经元培养物。使用甲基四唑鎓测定法评估暴露于0.01、0.1或1 mg / ml异丙酚长达48小时的细胞活力。为了评估γ-氨基丁酸-A（GABAA）受体的作用，还将细胞与GABAA受体拮抗剂，丙巴嗪和微毒素一起预孵育。 12小时后，浓度为1 mg / ml的丙泊酚显着降低了细胞活力。相反，该浓度导致3和6 h细胞活力显着增加。 GABAA受体拮抗剂不影响丙泊酚的神经变性作用，但取消了其神经保护作用。异丙酚处理24小时后，DNA片段化作为细胞凋亡的标志物升高。这些结果证实，高剂量的异丙酚可引起GABAA受体触发的神经保护作用，并随后在初级皮层神经元中引起时间依赖性但非GABAA依赖性的神经变性。

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