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首页> 外文期刊>Journal of International Medical Research >Identification of Three Strains of Mycobacterium Species Isolated from Clinical Samples Using Fatty Acid Methyl Ester Profiling
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Identification of Three Strains of Mycobacterium Species Isolated from Clinical Samples Using Fatty Acid Methyl Ester Profiling

机译:使用脂肪酸甲基酯谱分析从临床样品中分离出三株分枝杆菌种

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The cellular fatty acid profiles of 67 strains belonging to three different species of the genus Mycobacterium were determined by gas chromatography of the fatty acid methyl esters, using the MIDI Sherlock? Microbial Identification System (MIS). The species M. tuberculosis, M. xenopi and M. avium complex were clearly distinguishable and could be identified based on the presence and concentrations of 12 fatty acids: 14:0, 15:0, 16:1ω7c, 16:1ω6c, 16:0, 17:0, 18:2ω6,9c, 18:1ω9c, 18:0, 10Me-18:0 tuberculostearic acid, alcohol and cyclopropane. Fatty acid analysis showed that there is great homogeneity within and heterogeneity between Mycobacterium species. Thus the MIS is an accurate, efficient and relatively rapid method for the identification of mycobacteria.
机译:使用MIDI SherlockTM通过脂肪酸甲酯的气相色谱法测定了分枝杆菌属三个不同种的67个菌株的细胞脂肪酸谱。微生物识别系统(MIS)。结核分枝杆菌,异种分枝杆菌和鸟分枝杆菌复杂的物种可以清楚地区分,并且可以根据以下12种脂肪酸的存在和浓度进行鉴定:14:0、15:0、16:1ω7c,16:1ω6c,16: 0,17:0,18:2ω6,9c,18:1ω9c,18:0,10Me-18:0结核硬脂酸,醇和环丙烷。脂肪酸分析表明,分枝杆菌属之间有很大的同质性和异质性。因此,MIS是鉴定分枝杆菌的准确,有效和相对快速的方法。

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