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首页> 外文期刊>Journal of International Medical Research >Analysis of serum alpha-fetoprotein (AFP) and AFP-L3 levels by protein microarray
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Analysis of serum alpha-fetoprotein (AFP) and AFP-L3 levels by protein microarray

机译：通过蛋白质微阵列分析血清甲胎蛋白（AFP）和AFP-L3水平

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Objectives This study aimed to examine a simple, effective, time-saving, and low-cost protein microarray method for detecting serum alpha-fetoprotein (AFP) and AFP-L3 levels. Methods Serum samples from patients with hepatocellular carcinoma (HCC) (n?=?33) and control subjects (n?=?39) were collected and evaluated for the presence of AFP using a novel protein microarray. Glycoprotein (including AFP-L3) was enriched from crude samples by a Hotgen Biotech glycosyl capture spin column and then detected by protein microarray. An electrochemiluminescence immunoassay (ECLIA) was used to validate the measured values. Results Neither AFP levels lower than 20 ng/mL in the HCC group nor AFP levels higher than 20 ng/mL in the control group were found when tested by the ECLIA and protein microarray. The kappa test showed good consistency in the diagnostic performance of measuring serum AFP levels and the percentage of AFP-L3 in total AFP by the ECLIA and protein microarray. Protein microarray had advantages of smaller sample size required, low cost, and convenience compared with the ECLIA. Conclusion The protein microarray assay that was developed in the present study shows potential as an economic and convenient technique for detecting AFP and AFP-L3 levels in serum samples from patients with HCC.
机译：目的本研究旨在探讨一种简单，有效，省时且低成本的蛋白质微阵列方法，用于检测血清甲胎蛋白（AFP）和AFP-L3水平。方法收集肝细胞癌（HCC）患者（n = 33）和对照受试者（n == 39）的血清样品，并使用新型蛋白质微阵列评估AFP的存在。通过Hotgen Biotech糖基捕获旋转柱从粗样品中富集糖蛋白（包括AFP-L3），然后通过蛋白质微阵列检测。电化学发光免疫分析法（ECLIA）用于验证测量值。结果通过ECLIA和蛋白质芯片检测时，在肝癌组中均未发现AFP水平低于20 ng / mL，在对照组中未发现AFP水平高于20 ng / mL。 kappa测试显示通过ECLIA和蛋白质微阵列测量血清AFP水平和AFP-L3在总AFP中的百分比的诊断性能具有良好的一致性。与ECLIA相比，蛋白质微阵列的优点是所需样本量较小，成本低廉且方便。结论本研究开发的蛋白质微阵列测定法显示了一种经济，便捷的技术，可用于检测HCC患者血清中的AFP和AFP-L3水平。

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