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首页> 外文期刊>Journal of International Medical Research >4-1BB Ligand-Mediated Imbalance of Helper 17 T Cells and Regulatory T Cells in Patients with Allergic Asthma
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4-1BB Ligand-Mediated Imbalance of Helper 17 T Cells and Regulatory T Cells in Patients with Allergic Asthma

机译：过敏性哮喘患者中4-1BB配体介导的辅助性17 T细胞和调节性T细胞失衡

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OBJECTIVES: To investigate the presence of 4-1BB ligand (4-1BBL) in the peripheral blood of patients with allergic asthma and evaluate its role in controlling the balance between helper 17 T (Th17) and regulatory T (Treg) cells. METHODS: Soluble 4-1BBL (s4-1BBL) was quantified by enzyme-linked immunosorbent assay in plasma from patients with asthma (n = 45) and from healthy control subjects (n = 35). The proportion of monocytes positive for membrane-bound 4-1BBL (m4-1BBL) was determined by flow cytometry. Peripheral blood mononuclear cells from patients with asthma were incubated with anti-4-1BB monoclonal antibody in vitro. Concentrations of interleukin (IL)-17 and transforming growth factor (TGF)-β1 in the culture supernatant were analysed. RESULTS: Plasma s4-1BBL concentrations and the proportion of m4-1BBL-positive monocytes were significantly lower in patients with asthma than in control subjects. The culture supernatant concentration of TGF-β1 was increased and that of IL-17 was decreased by incubation with anti-4-1BB monoclonal antibody. CONCLUSIONS: Both soluble and membrane-bound 4-1BBL were reduced in patients with allergic asthma compared with control subjects. 4-1BBL/4-1BB signalling may play an important role in allergic asthma by regulating the Th17/Treg balance.
机译：目的：调查过敏性哮喘患者外周血中4-1BB配体（4-1BBL）的存在，并评估其在控制辅助17 T（Th17）和调节性T（Treg）细胞之间的平衡的作用。方法：通过酶联免疫吸附法对哮喘患者（n = 45）和健康对照组（n = 35）的血浆中可溶性4-1BBL（s4-1BBL）进行定量。通过流式细胞术确定膜结合的4-1BBL（m4-1BBL）阳性的单核细胞比例。将来自哮喘患者的外周血单核细胞与抗4-1BB单克隆抗体进行体外孵育。分析培养上清液中白介素（IL）-17和转化生长因子（TGF）-β1的浓度。结果：哮喘患者的血浆s4-1BBL浓度和m4-1BBL阳性单核细胞比例明显低于对照组。通过与抗4-1BB单克隆抗体孵育，可提高TGF-β1的培养上清液浓度，降低IL-17的浓度。结论：与对照组相比，过敏性哮喘患者的可溶性和结合膜的4-1BBL均降低。 4-1BBL / 4-1BB信号传导可能通过调节Th17 / Treg平衡在过敏性哮喘中发挥重要作用。

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