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首页> 外文期刊>Journal of International Medical Research >Magnetic resonance imaging characteristics of hepatocyte apoptosis (induced by right portal vein ligation) and necrosis (induced by combined right portal vein and right hepatic artery ligation) in rats
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Magnetic resonance imaging characteristics of hepatocyte apoptosis (induced by right portal vein ligation) and necrosis (induced by combined right portal vein and right hepatic artery ligation) in rats

机译：大鼠肝细胞凋亡（由右门静脉结扎引起）和坏死（由右门静脉和右肝动脉结扎引起）的磁共振成像特征

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Objective Using magnetic resonance imaging (MRI), this study aimed to demonstrate the appearance of hepatocytes following ligation of the right portal vein or combined ligation of the right portal vein and right hepatic artery, in a rat model. Methods Ninety adult Sprague–Dawley rats (body weight 250–300?g) were divided into three groups (n?=?30 per group): ligation of the right portal vein (Group A); combined ligation of the right portal vein and right hepatic artery (Group B); no intervention to obstruct blood supply (control group). Rats were then randomly subdivided into five groups that underwent examination at 3?h and 1, 3, 7 and 14?days postprocedure (n?=?6 per group). Livers were examined by routine MRI, diffusion-weighted imaging (DWI) and magnetic resonance spectroscopy (MRS). After examination, each group of rats was sacrificed. Right hepatic lobes were removed and examined by pathology in six rats per timepoint; transmission electron microscopy (TEM) was undertaken in up to three rats per timepoint. Results Hepatocyte apoptosis and necrosis, by right portal vein ligation and combined right portal vein and right hepatic artery ligation respectively, were confirmed by pathology and TEM. In Group A, there were slight increases in intensity of T1- and T2-weighted images and in the apparent diffusion coefficient (ADC). In addition there were larger areas under the lactic acid and lipid peaks on MRS. In Group B, there were increased T1 and T2 signals, a decrease in the ADC and an increase of the area under the lactic acid peaks on MRS. Conclusions Hepatocyte apoptosis and necrosis induced by ligation procedures exhibited specific changes that were evident on MRI, DWI and MRS, and could be detected in?vivo by MRI, in a rat model.
机译：目的使用磁共振成像（MRI），该研究旨在证明在大鼠右门静脉结扎或右门静脉与右肝动脉联合结扎后肝细胞的出现。方法将成年成年Sprague-Dawley大鼠（体重250-300？g）分为三组（每组n？=？30）：结扎右门静脉（A组）;每组30只。右门静脉和右肝动脉联合结扎（B组）;没有干预以阻止血液供应（对照组）。然后将大鼠随机分为五组，分别在手术后3小时，1、3、7和14天进行检查（每组n = 6）。通过常规MRI，弥散加权成像（DWI）和磁共振波谱学（MRS）检查肝脏。检查后，处死每组大鼠。取出右肝叶并通过病理学在每个时间点对六只大鼠进行病理检查。每个时间点最多对三只大鼠进行透射电子显微镜（TEM）。结果分别经右门静脉结扎，右门静脉和右肝动脉联合结扎，证实了肝细胞的凋亡和坏死。在A组中，T1和T2加权图像的强度和表观扩散系数（ADC）略有增加。此外，MRS上的乳酸和脂质峰下方有较大的区域。在B组中，T1和T2信号增加，ADC减少，MRS上乳酸峰下方的面积增加。结论结扎过程诱导的肝细胞凋亡和坏死表现出特异性变化，在MRI，DWI和MRS上很明显，并且可以在MRI体内检测到。

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