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首页> 外文期刊>Journal of King Saud University >Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No. AB184139) isolated from Indian soil
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Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No. AB184139) isolated from Indian soil

机译：从印度土壤中分离的灰链霉菌（保藏号：AB184139）产生的纤维素分解酶的表征

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摘要

The present study was intended to investigate the effect of different physico-chemical parameters that included pH (3–11), temperature (4–60 °C), incubation period (2–8 days) and NaCl (1–10% w/v) concentration on the growth and cellulase enzyme production of the actinomycete strain St-1, to optimize its enzyme productivity. The strain St-1 isolated locally from soil sample and identified as Streptomyces griseorubens at MTCC and Gene Bank, Chandigarh, India (Accession No. AB184139), showed an optimum growth at pH 7, temperature of 45 °C and 6 days of incubation period. The cellulose hydrolysis by the isolate was also optimum at these parameters when the maximum level of reducing sugar produced due to filter paper activity was 5.6 mg per mL and CMC activity was 4.5 mg per mL. The isolate was moderately halophilic, as it was unable to grow beyond 6% of NaCl (w/v) concentration.
机译：本研究旨在研究不同物理化学参数的影响，包括pH（3-11），温度（4-60°C），潜伏期（2-8天）和NaCl（1-10％w / v）集中放线菌菌株St-1的生长和纤维素酶的产生，以优化其酶的生产率。从土壤样品中局部分离出的St-1菌株在印度昌迪加尔的MTCC和Gene Bank（登录号AB184139）上被鉴定为灰链霉菌（Streptomyces griseorubens）（保藏号AB184139），在pH 7，温度45°C和孵育期6天时表现出最佳生长。当由于滤纸活性产生的最大还原糖水平为5.6 mg / mL和CMC活性为4.5 mg / mL时，分离物的纤维素水解在这些参数下也是最佳的。该分离物具有中等嗜盐性，因为它无法生长超过NaCl（w / v）浓度的6％。

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