...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of International Medical Research >Identification of potential long noncoding RNA associated with nasopharyngeal carcinoma using deep sequencing
【24h】

Identification of potential long noncoding RNA associated with nasopharyngeal carcinoma using deep sequencing

机译：使用深度测序鉴定与鼻咽癌相关的潜在长非编码RNA

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective To identify differentially expressed long noncoding RNAs (lncRNAs) in nasopharyngeal carcinoma (NPC) compared with chronic nasopharyngitis (CNP) tissues. Methods This prospective cohort study enrolled patients with NPC and CNP. The levels of lncRNAs in NPC and CNP tissues was detected by deep sequencing and quantitative polymerase chain reaction. Kyoto Encyclopedia of Genes and Genomes pathway analysis and antisense prediction were performed to reveal the function of differentially expressed lncRNAs (DELs). Receiver operating characteristic (ROC) curve and correlation analyses were used to evaluate the clinical and prognostic value of lncRNAs. Results A total of 30 NPC and 27 CNP tissues were analysed. A total of 296 DELs were identified. LncRNAs ENSG00000227084 and ENSG00000230489 might play important roles in the development of NPC by interfering with the Rap1 signalling pathway and natural killer cell-mediated cytotoxicity, respectively. Antisense prediction showed that lncRNA ENSG00000230489 was paired with VAV3 mRNA. LncRNAs ENSG00000230489 (area under the ROC curve?=?0.9138) and ENSG00000227084 (area under the ROC curve?=?0.8037) may be diagnostic markers for NPC. Furthermore, low levels of ENSG00000230489 was positively associated with distant metastasis. Conclusion LncRNAs ENSG00000230489 and ENSG00000227084 may be potential diagnostic markers and therapeutic targets for NPC.
机译：目的鉴定鼻咽癌（NPC）与慢性鼻咽炎（CNP）组织中差异表达的长非编码RNA（lncRNA）。方法这项前瞻性队列研究纳入了NPC和CNP患者。通过深度测序和定量聚合酶链反应检测NPC和CNP组织中lncRNA的水平。进行了《京都基因与基因组百科全书》通路分析和反义预测，以揭示差异表达的lncRNA（DELs）的功能。接收者操作特征（ROC）曲线和相关分析被用来评估lncRNA的临床和预后价值。结果共分析了30例NPC和27例CNP组织。总共鉴定出296个DEL。 LncRNA ENSG00000227084和ENSG00000230489可能分别通过干扰Rap1信号通路和自然杀伤细胞介导的细胞毒性在NPC的发展中发挥重要作用。反义预测表明lncRNA ENSG00000230489与VAV3 mRNA配对。 LncRNAs ENSG00000230489（ROC曲线下的面积≤0.9138）和ENSG00000227084（ROC曲线下的面积≤0.8037）可能是NPC的诊断标志物。此外，低水平的ENSG00000230489与远处转移呈正相关。结论LncRNA ENSG00000230489和ENSG00000227084可能是鼻咽癌的潜在诊断标志和治疗靶点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号