...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of King Saud University >Histopathological changes and expression of transforming growth factor beta (TGF-β3) in mice exposed to gliotoxin
【24h】

Histopathological changes and expression of transforming growth factor beta (TGF-β3) in mice exposed to gliotoxin

机译：胶质毒素暴露小鼠的组织病理学变化和转化生长因子β（TGF-β3）的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundGliotoxin (GT) is the prototype of a class of epipolythiodioxopiperazines (ETPs) which are secondary metabolites made by fungi organisms only e.g.,Aspergillus fumigatus,characterized by a disulfide bridge across a piperazine ring with low molecular weight (326?Da),and its sulphur bridge imparts all known toxicity of these molecules. Its name was derived from its identification as a metabolite ofGliocladium fimbriatumas it is previously isolated. It had histopathological effects of human and animals tissues and thus may alter the immune response.ObjectivesThe current study aimed to evaluate the effect of GT on immunological and histological changes in lung, liver, and kidney in addition to morphological changes in colon using animal models.MethodologyMature male BALB/c mice were used in this study, animals were provided by Biotechnology researches center of Al-Nahrain University. To study the histological changes and expression of Transforming growth factor beta (TGF-β3) in mice, animals were injected intraperitoneally (i.p.) with acute dose of both sample and standard gliotoxin with control group (received i.p. one dose of methanol 10%) and the mice of each group were sacrificed at day seven, each mouse was dissected and the organs lung, liver and kidney were collected then sectioned. Mice of another groups were administrated with one dose of sample gliotoxin through the lumen of the colon using a vinyl catheter positioned 5?cm from the anus, and the control group received methanol 10%, the mice were killed on day seven of administration to study the morphological changes in the colon.ResultsGross-examination showed clear pathological changes in examined organs of mice treated intraperitoneally with gliotoxin (i.p.) injection during seven days, lesions were seen in examined organs with groups of all concentrations of both sample and standard gliotoxin in comparing with control. After intrarectal administration, many signs for mycotoxicosis were observed, like shivering, redness around the anus and bristling of hair. Although of the aggressive behavior of mice and loss of activity at the day 6 of treatment was also reduced. In both sample and standard GT groups, the elevation at the high concentrations of the gliotoxin gave over TGF-β3 expression in liver, lung, and kidney.ConclusionsIt can be concluded that both of sample and standard gliotoxin showed the same effectivenessin vivoto induce histopathological changes and the immunohistochemical studies revealed that the increasing of TGF-β3 expression was in a significant relationship between the immunoreactive cell and its intensity with number and size of lesion in the same tissue at probability (P value)?
机译：背景胶体毒素（GT）是一类表聚硫代二氧杂哌嗪（ETP）的原型，它们是仅由真菌生物体（例如烟曲霉）形成的次级代谢产物，其特征是通过二硫键跨过低分子量（326？Da）的哌嗪环而形成。硫桥赋予这些分子所有已知的毒性。它的名字源于它以前被分离出的鉴定为纤维毛胶质瘤的代谢产物。它具有人类和动物组织的组织病理学效应，因此可能会改变免疫反应。目的当前的研究旨在通过动物模型评估GT对结肠，结肠和肝脏形态学变化的免疫学和组织学变化的影响。方法：本研究使用成熟的雄性BALB / c小鼠，动物由Al-Nahrain大学生物技术研究中心提供。为了研究小鼠的组织学变化和转化生长因子β（TGF-β3）的表达，给动物腹膜内（ip）注射急性剂量的样品和标准胶质毒素，并与对照组进行腹膜内注射（ip接受10％甲醇）。在第7天处死每组小鼠，解剖每只小鼠，收集器官，肺，肝和肾，然后切片。使用距肛门5？cm的乙烯基导管，将另一剂量的小鼠胶质毒素通过结肠腔给药另一组小鼠，对照组接受10％的甲醇，在给药的第7天将小鼠处死进行研究结果体格检查显示，在7天的时间里，腹腔注射gliotoxin（ip​​）注射的小鼠腹腔内检查的器官有明显的病理变化，在所有浓度的标本和标准gliotoxin的组中都观察到了病变与控制。直肠内给药后，观察到许多霉菌毒素中毒迹象，例如发抖，肛门周围发红和头发硬毛。尽管具有小鼠的攻击行为并且在治疗的第6天时活性降低也减少了。在标本和标准GT组中，高浓度的胶质毒素在肝，肺和肾脏中的表达都超过了TGF-β3的表达。结论可以得出结论，标本和标准胶质毒素在体内诱导组织病理学改变的效果相同。免疫组织化学研究表明，同一组织中TGF-β3表达的增加与免疫反应细胞的强度及其强度与病变的数量和大小呈显着相关性（P值）≤0.05，因此有待进一步研究需要评估胶质毒素对其他细胞因子的急性和/或慢性作用，还需要进一步组织病理学研究胶质毒素对其他器官，例如肌肉，心脏，大脑，脾脏和肠道的急性和/或慢性作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号