...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of King Saud University >Influence of light wavelengths, light intensity, temperature, and pH on biosynthesis of extracellular and intracellular pigment and biomass of Pseudomonasaeruginosa NR1
【24h】

Influence of light wavelengths, light intensity, temperature, and pH on biosynthesis of extracellular and intracellular pigment and biomass of Pseudomonasaeruginosa NR1

机译：光波长，光强度，温度和pH值对 Pseudomonasaeruginosa NR1 的细胞外和细胞内色素和生物量生物合成的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of the present study was to check whether the light is an inducing agent or not for the intracellular and extracellular pigment production byPseudomonas aeruginosa NR1? Is the produced pigment a photoprotective in nature? The aim of the present research study was to evaluate the influence of different light wavelengths, light intensity, temperature, and pH on biomass and intracellular and extracellular pigment production. A strain ofPseudomonasthat was thermoalkalophilic and produces red non-diffusible intracellular and green-diffusible extracellular pigment was isolated, until now no one has reported this type of strain. For determining the effect of different light wavelengths, we introduced and designed a novel ‘selective light wavelength method’, which gave us better results than previously used glass paper wrapping method. Extracellular pigment production was found maximum under darkness, red and blue light wavelengths. Same results were obtained in glass paper wrapping method, but the concentration of pigment produced by bacteria was greater in our method which suggests us that the ‘selective light wavelength method’ is better (for pigment yield) between these methods.The outcome of this study refutes the photo protective role of extracellular pigment, claimed by many researchers. Red and light wavelength was found to suppress the intracellular pigment production. Extracellular pigment production byPseudomonas aeruginosa NR1was maximum in darkness with 4.042?±?0.03?OD/mL and minimum in green light (Glass paper plates (GPP), 2.151?±?0.24?OD/mL; Selective lamp plates (SLP), 2.232?±?0.28?OD/mL). Maximum intracellular pigment (SLP, 4.672?±?0.15?OD/mL; GPP, 4.395?±?0.03?OD/mL) and biomass concentrations (Selective lamp method-830?±?5.7?μg/0.01?L; Glass paper method, 810?±?2.88?μg/0.01?L) were achieved under yellow light wavelength. Minimum intracellular pigment yield (GPP, 2.124?±?0.01?OD/mL; SLP, 2.252?±?0.0?OD/mL and biomass yield (Glass paper method, 363.3?±?3.33?μg/0.01?L; selective lamp method-385?±?2.88?μg/0.01?L) was recorded under red light wavelength. Direct relationship was observed between biomass production and intracellular pigment production. Maximum extracellular (3.548?±?2.2?OD/mL) and intracellular pigment (3.856?±?0.02?OD/mL) and biomass concentration (800?±?1.44?μg/100?mL) were observed when pH was 7. The intracellular pigment concentration (3.698?±?0.01?OD/ml) and biomass production (656?±?3.05?μg/ml) were maximal at a temperature of 30?°C. The maximum concentration of extracellular pigment was recorded at 35?°C (3.924?±?0.04?OD/ml).
机译：本研究的目的是检查光是否是铜绿假单胞菌NR1产生细胞内和细胞外色素的诱导剂？生产的颜料本质上具有光保护性吗？本研究的目的是评估不同光波长，光强度，温度和pH对生物量以及细胞内和细胞外色素生成的影响。分离出一种嗜热假单胞菌菌株，该菌株是嗜热嗜碱性的，并产生红色的不可扩散的细胞内和绿色的扩散性细胞外色素，直到现在还没有人报道这种类型的菌株。为了确定不同光波长的影响，我们引入并设计了一种新颖的“选择性光波长方法”，与以前使用的玻璃纸包装方法相比，它可以提供更好的结果。发现在黑暗，红色和蓝色光波长下，细胞外色素产生最大。在玻璃纸包装方法中也获得了相同的结果，但是在我们的方法中细菌产生的色素浓度更高，这表明在这两种方法之间，``选择性光波长法''更好（对于色素产量而言）。许多研究人员声称，这种说法反驳了细胞外色素的光保护作用。发现红色和光波长抑制细胞内色素的产生。铜绿假单胞菌NR1产生的细胞外色素在黑暗中最大，在4.042？±？0.03？OD / mL下，在绿光下最少（玻璃板（GPP），2.151？±？0.24？OD / mL;选择性灯板（SLP），2.232 ≤±0.28≤OD/ mL）。最大细胞内色素（SLP，4.672？±？0.15？OD / mL; GPP，4.395？±？0.03？OD / mL）和生物质浓度（Selective lamp method-830？±？5.7？μg/ 0.01？L;玻璃纸方法，在黄光波长下获得810±±2.88μg/0.01μL。最小细胞内色素产量（GPP，2.124？±？0.01？OD / mL; SLP，2.252？±？0.0？OD / mL和生物质产量（玻璃纸法，363.3？±？3.33？μg/ 0.01？L;选择性灯method-385？±？2.88？g / 0.01？L）在红光波长下记录，观察到生物量的产生与细胞内色素的产生之间的直接关系，最大的细胞外（3.548？±？2.2？OD / mL）和细胞内的色素（ pH值为7时，观察到3.856？±？0.02？OD / mL和生物质浓度（800？±？1.44？μg/ 100？mL）。在30°C时最大产量（656±±3.05μg/ ml）。细胞外色素的最大浓度在35°C（3.924±±0.04OD / ml）下记录。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号