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首页> 外文期刊>Journal of King Saud University >Cytotoxicity and genotoxicity of gliotoxin on human lymphocytes in vitro
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Cytotoxicity and genotoxicity of gliotoxin on human lymphocytes in vitro

机译：胶质毒素对人淋巴细胞的细胞毒性和遗传毒性体外

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The cytotoxic effects on human lymphocytes of two gliotoxin samples (one pure sample produced in the laboratory for this study, and one sample purchased from a standard source) were assessed at four different concentrations (25, 50, 100 and 200 ng/ml) using the methylthiazol tetrazolium (MTT) bioassay. The results showed that growth was inhibited by 21, 39.10, 61.99 and 87.45% for each of the four concentrations of the pure sample, respectively, and by 17.89, 34.92, 58.34 and 85.22% respectively, in the case of the standard purchased sample. Deoxyribonucleic acid (DNA) was extracted from the lymphocytes and analysed by electrophoresis on a 1% agarose gel. Gliotoxin appeared to have the ability to degrade or damage the DNA. The present study showed that both the growth inhibition and DNA damage experienced by the human lymphocytes increased linearly with increasing concentrations of toxin.
机译：使用以下四种浓度（25、50、100、200 ng / ml）评估了两种胶质毒素样品（一种用于本研究的实验室生产的纯样品，一种是从标准来源购买的样品）对人淋巴细胞的细胞毒性作用。甲基噻唑四唑（MTT）生物测定。结果表明，对于四种浓度的纯样品，分别抑制了21、39.10、61.99和87.45％的生长，对于标准购买的样品，分别抑制了17.89、34.92、58.34和85.22％。从淋巴细胞中提取脱氧核糖核酸（DNA），并在1％琼脂糖凝胶上进行电泳分析。胶质毒素似乎具有降解或破坏DNA的能力。本研究表明，人淋巴细胞经历的生长抑制和DNA损伤均随着毒素浓度的增加而线性增加。

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