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首页> 外文期刊>Journal of King Saud University >Evaluation of SSR-based genetic diversity, protein and mineral content in black gram genotypes
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Evaluation of SSR-based genetic diversity, protein and mineral content in black gram genotypes

机译：基于黑社会基因型的基于SSR的遗传多样性，蛋白质和矿物质含量的评估

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Black gram [Vigna mungo(L.) Hepper] is an important food source which is rich in digestible good quality protein and many minerals. But the information on grain minerals and SSR marker based variability study is scanty. Therefore, to examine the variability for protein and minerals in black gram, 60 genotypes were screened for protein and three minerals (calcium, potassium and phosphorus) along with 11 SSR markers. The germplasm displayed ample variability for protein and minerals. The average value of protein, calcium, potassium and phosphorus content was 22.96%, 135.26?mg/100?g, 468.59?mg/100?g and, 325.96?mg/100?g. Phosphorus was significantly correlated with potassium in positive direction. A total of 66 alleles were identified from 60 black gram genotypes. The number of alleles per SSR marker was six with a range of 2–12. PIC value ranged from 0.30 (CEDG 92) to 0.90 (Vmg SSR 29 and Vmg SSR 53) with average PIC of 0.60. The major allelic frequency ranged from 0.13 to 0.78. The minimum and maximum molecular distance were found to be 0.09–1.00. The average genetic distance between 60 black gram genotypes was 0.69. The current experiment indicated that the studied genotypes exhibited ample genetic variability at biochemical and DNA level and can be used for black gram improvement during selection and hybridization program.
机译：黑克[Vigna mungo（L。）Hepper]是重要的食物来源，富含可消化的优质蛋白质和许多矿物质。但是基于谷物矿物和基于SSR标记的变异性研究的信息很少。因此，为了检查黑克蛋白质和矿物质的变异性，筛选了60个基因型的蛋白质和三种矿物质（钙，钾和磷）以及11个SSR标记。种质对蛋白质和矿物质显示出足够的变异性。蛋白质，钙，钾和磷的平均值分别为22.96％，135.26？mg / 100？g，468.59？mg / 100？g和325.96？mg / 100？g。磷与钾在正方向上显着相关。从60个黑克基因型中总共鉴定出66个等位基因。每个SSR标记的等位基因数量为6，范围为2-12。 PIC值范围从0.30（CEDG 92）到0.90（Vmg SSR 29和Vmg SSR 53），平均PIC为0.60。主要等位基因频率范围为0.13至0.78。发现最小和最大分子距离为0.09–1.00。 60个黑克基因型之间的平均遗传距离为0.69。目前的实验表明，所研究的基因型在生化和DNA水平上具有足够的遗传变异性，可用于选择和杂交程序中的黑革菌改良。

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