...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of King Saud University >Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer
【24h】

Exosomal miRNA-93 and miRNA-205 expression in endometrial cancer

机译：子宫内膜癌中外泌体miRNA-93和miRNA-205的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Endometrial Carcinoma (EC) is one of the most common cancers of females’ reproductive system. It has been given first rank in the tumors of obstetrics and gynaecology and day by day the incidence of EC gradually increased in recent years, which has critically harmed the woman health. 100 cases of Endometrial cancer and equal number of female healthy subjects were included and serum were separated from blood sample collected in plain vials from all study participant. Total RNA was extracted from exosomes extracted from serum and cDNA were synthesised from 100?ng of total RNA. cDNA were subjected to quantitative real time PCR to study the microRNA-95 and micro-RNA205 expression among cased and controls using Taqman assay. 3.78 mean fold increased exosomalmicroRNA-95 expression was observed while 0.07 mean fold decreased exosomal microRNA was found in endometrial cancer patients. Increased expression of exosomal miRNA-93 expression were linked with smoking (p?=?0.002), grade of tumor (p?=?0.01), FIGO stage (p?
机译：子宫内膜癌（EC）是女性生殖系统最常见的癌症之一。它在妇产科肿瘤中被列为第一位，近年来，EC的发病率逐渐增加，严重危害了妇女的健康。包括100例子宫内膜癌和相等数量的女性健康受试者，并从所有研究参与者的普通小瓶中采集的血液样本中分离血清。从血清中提取的外来体中提取总RNA，并从100ng的总RNA中合成cDNA。使用Taqman分析法对cDNA进行定量实时PCR，以研究病例和对照组之间的microRNA-95和microRNA205表达。在子宫内膜癌患者中，外泌体microRNA-95表达平均增加了3.78倍，而外泌体microRNA平均减少了0.07倍。外泌体miRNA-93表达的增加与吸烟（p？=？0.002），肿瘤的分级（p？=？0.01），FIGO分期（p？<？0.0001）和远处器官转移（p？<？0.0001）有关。 ）。与健康对照组相比，在EC患者中观察到外泌体miRNA-205的miRNA-205表达降低（平均倍数为0.07倍），并发现与吸烟有关（p？=？0.01），淋巴结受累（p？=？0.001）， FIGO级（p≤0.004）。观察到总体中位生存期差与子宫内膜癌患者的外泌体microRNA-95升高和microRNA-205降低有关。 ROC曲线显示，基于血清的外泌体microRNA-93表达，外泌体miRNA-93的截止值增加了3.25倍，敏感性和特异性分别为93％和97％（AUC为0.99，p≤<0.0001）。研究发现外泌体microRNA-93与外泌体microRNA-205表达呈负相关（r？=？0.20，p？=？0.04）。研究得出结论，外泌体microRNA-95和外泌体microRNA-205与疾病恶化有关，并且可能是子宫内膜癌患者的预后指标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号