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首页> 外文期刊>Journal of Krishna Institute of Medical Sciences University. >Detection of ?-Lactamase Activity in Various Clinical Bacterial Isolates by Three Different Methods And its Correlation with Drug Resistance
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Detection of ?-Lactamase Activity in Various Clinical Bacterial Isolates by Three Different Methods And its Correlation with Drug Resistance

机译：三种不同方法检测各种临床细菌分离物中β-内酰胺酶活性及其与耐药性的关系

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Background: β-lactams such as penicillins arethe most widely used antibiotics, and β-lactamases are the greatest source of resistanceto penicillins. Aims and Objectives: To studyβ-lactamase production in clinical isolates offamily Enterobacteriaceae, P. aeruginosa andStaphylococci by three different methods andto correlate its potential with drug resistance;with an endeavour to evaluate convenient andeconomical method duly supported by relevantMinimum Inhibitory Concentration (MIC) stud-ies. Material and Methods: Total 240 clinicalisolates (Gram-negative bacilli-191, staphylo-cocci-49) were subjected to antimicrobial sus-ceptibility testing by Kirby-Bauer disk diffu-sion method and MIC for ampicillin and peni-cillin was determined by agar dilution method.β-lactamase was detected by broth acidometric,iodometric cell suspension and microbiologi-cal method. Results: Multidrug resistance wasobserved in more than 90% isolates. One hun-dred and ninety Gram-negative bacilli were re-sistant to ampicillin and 47 staphylococcal iso-lates were resistant to both penicillin and ampi-cillin. Though microbiological method gavehighest positive results 210 (87.5%),iodometric method could detect β-lactamasein apparently sensitive isolates as well givingsatisfactory [207 (86.25%)] comparable re-sults. Conclusion: In view of the noted bacte-rial resistance, tests for β-lactamase should becarried out on a routine basis for an early imple-mentation of appropriate antimicrobial therapy.Iodometric method is eminently convenient,economical and reliable method. Isolates show-ing MIC 0.125μg/ml for penicillin and MIC8μg/ml for ampicillin should be checked forβ-lactamase production.
机译：背景：诸如青霉素之类的β-内酰胺类药物是使用最广泛的抗生素，而β-内酰胺酶是对青霉素类药物产生耐药性的最大来源。目的和目的：通过三种不同的方法研究临床分离的肠外科肠杆菌科细菌，铜绿假单胞菌和葡萄球菌中β-内酰胺酶的产生，并将其潜力与耐药性相关联;努力评估相关最低抑菌浓度（MIC）标准支持下的便捷经济方法ies。材料与方法：对总计240株临床分离株（革兰氏阴性杆菌191，葡萄球菌49）通过Kirby-Bauer纸片扩散法进行了药敏试验，并通过琼脂法测定了氨苄青霉素和青霉素的MIC稀释法。用肉汤酸度法，碘量法细胞悬浮液和微生物法检测β-内酰胺酶。结果：在超过90％的分离物中观察到多药耐药性。一百零九十个革兰氏阴性杆菌对氨苄西林耐药，而47个葡萄球菌同工型对青霉素和氨苄西林均具有抗性。尽管微生物方法获得了最高的阳性结果210（87.5％），但碘量法可以检测到β-内酰胺酶表面上敏感的分离物，并且结果令人满意[207（86.25％）]。结论：鉴于明显​​的细菌耐药性，应常规进行β-内酰胺酶测试，以尽早实施适当的抗菌治疗。碘量法是一种方便，经济且可靠的方法。对于青霉素，应检查其MIC <0.125μg/ ml，氨苄青霉素的MIC <8μg/ ml。

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