...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Korean medical science. >Norcantharidin Induces Human Melanoma A375-S2 Cell Apoptosis through Mitochondrial and Caspase Pathways
【24h】

Norcantharidin Induces Human Melanoma A375-S2 Cell Apoptosis through Mitochondrial and Caspase Pathways

机译：Norcantharidin通过线粒体和胱天蛋白酶途径诱导人黑色素瘤A375-S2细胞凋亡。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Norcantharidin (NCTD) is the demethylated form of cantharidin, which is the active substance of mylabris. To examine the pathway of NCTD-induced A375-S2 cell death, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-dipheyltetrazolium bromide (MTT) assay, photomicroscopical observation, DNA agarose gel electrophoresis, caspase activity assay and Western blot analysis were carried out. A375-S2 cells treated with NCTD exhibited several typical characteristics of apoptosis. The inhibitory effect of NCTD on human melanoma, A375-S2 cells, was partially reversed by the inhibitors of pan-caspase, caspase-3 and caspase-9. The activities of caspase-3 and -9 were significantly increased after treatment with NCTD at different time. The expression of inhibitor of caspase-activated DNase was decreased in a time-dependent manner, simultaneously, the ratio of Bcl-2/Bax or Bcl-xL/Bax was decreased and the expression ratio of proteins could be reversed by caspase-3 inhibitor. The expression of cytochrome c in cytosol was increased after NCTD treatment and caspase-3 inhibitor had no significant effect on the up-regulation of cytochrom c . These results suggest that NCTD induced A375-S2 cell apoptosis and the activation of caspase and mitochondrial pathway were involved in the process of NCTD-induced A375-S2 cell apoptosis.
机译：Norcantharidin（NCTD）是Cantharidin的去甲基化形式，它是Mylabris的活性物质。若要检查NCTD诱导的A375-S2细胞死亡的途径，需要进行3-（4，5-二甲基噻唑-2-基）-2、5-二苯甲基四唑溴化物（MTT）测定，光学显微镜观察，DNA琼脂糖凝胶电泳，胱天蛋白酶活性测定进行蛋白质印迹分析。 NCTD处理的A375-S2细胞表现出几种典型的凋亡特征。泛半胱天冬酶，caspase-3和caspase-9抑制剂可部分逆转NCTD对人黑素瘤A375-S2细胞的抑制作用。在不同时间用NCTD处理后，caspase-3和-9的活性显着增加。半胱天冬酶激活的DNase抑制剂的表达呈时间依赖性降低，同时，Bcl-2 / Bax或Bcl-x L / Bax的比例降低，蛋白质的表达比例降低。可以被caspase-3抑制剂逆转。 NCTD处理后胞浆中细胞色素c的表达增加，而caspase-3抑制剂对细胞色素c的上调无明显影响。这些结果表明NCTD诱导的A375-S2细胞凋亡和胱天蛋白酶和线粒体途径的激活参与了NCTD诱导的A375-S2细胞凋亡的过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号