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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Induction of mycobacterial proteins during phagocytosis and heat shock: a time interval analysis.
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Induction of mycobacterial proteins during phagocytosis and heat shock: a time interval analysis.

机译：吞噬作用和热休克过程中分枝杆菌蛋白的诱导：时间间隔分析。

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摘要

Mycobacterium tuberculosis survives macrophage bactericidal activities by mechanisms that may include induction of stress proteins. We sought to determine whether the synthesis of any mycobacterial proteins is increased during phagocytosis and whether any of these proteins are also up-regulated during heat shock. Protein synthesis by M. tuberculosis H37Ra during phagocytosis by the mouse macrophage cell line IC-21, and during heat shock at 45 and 48 degrees C, was monitored at various time intervals using 35S-labeled methionine/cysteine and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data suggest the existence of certain common elements in the stress response of mycobacteria to the three stress stimuli. This apparent similarity was best characterized by the up-regulation of a 25-kDa protein after exposure to each of the stress conditions. Furthermore, this 25-kDa protein and a 37-kDa protein that was also synthesized during phagocytosis appeared to be extracellular because they were preferentially solubilized when infected macrophages were lysed with 0.5% NP-40.
机译：结核分枝杆菌通过可能包括诱导应激蛋白的机制在巨噬细胞杀菌活性中幸存下来。我们试图确定在吞噬作用期间是否增加了任何分枝杆菌蛋白质的合成，以及在热休克期间这些蛋白质中的任何一种是否也被上调。使用35S标记的蛋氨酸/半胱氨酸和十二烷基硫酸钠-聚丙烯酰胺凝胶在不同的时间间隔监视小鼠巨噬细胞IC-21吞噬作用期间以及在45和48摄氏度的热休克期间结核分枝杆菌H37Ra的蛋白质合成电泳。我们的数据表明，分枝杆菌对三种应激刺激的应激反应中存在某些共同的因素。这种明显的相似性最好通过暴露于每种胁迫条件后25-kDa蛋白的上调来表征。此外，在吞噬作用期间也合成的这种25 kDa蛋白和37 kDa蛋白似乎是细胞外的，因为当感染的巨噬细胞用0.5％NP-40裂解时，它们优先溶解。

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