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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Effects of macrophage colony-stimulating factor (M-CSF) on the development, differentiation, and maturation of marginal metallophilic macrophages and marginal zone macrophages in the spleen of osteopetrosis (op) mutant mice lacking functional M-CSF activity.
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Effects of macrophage colony-stimulating factor (M-CSF) on the development, differentiation, and maturation of marginal metallophilic macrophages and marginal zone macrophages in the spleen of osteopetrosis (op) mutant mice lacking functional M-CSF activity.

机译：巨噬细胞集落刺激因子（M-CSF）对缺乏功能性M-CSF活性的骨质疏松症（op）突变小鼠脾中边缘嗜金属巨噬细胞和边缘区巨噬细胞发育，分化和成熟的影响。

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Immunohistochemical techniques using an anti-mouse panmacrophage monoclonal antibody and anti-mouse monoclonal antibodies specific for marginal metallophilic macrophages or marginal zone macrophages were used to detect red pulp macrophages, marginal metallophilic macrophages, and marginal zone macrophages in the spleen of op/op mice. In the mutant mice, the red pulp macrophages were reduced to about 60% of those in the normal littermates and the marginal metallophilic macrophages and marginal zone macrophages were absent. After administration of recombinant human macrophage colony-stimulating factor (rhM-CSF), numbers of red pulp macrophages increased rapidly, reaching levels found in normal littermates 1 week later. In contrast, the marginal metallophilic macrophages as well as the marginal zone macrophages appeared slowly after rhM-CSF administration and their numbers were less than half of the baseline level of normal littermates even at 12 weeks of administration. The distribution of marginal metallophilic macrophages and marginal zone macrophages appearing after M-CSF administration was irregular in the spleen of the op/op mice. These splenic macrophage subpopulations differed in their responses to rhM-CSF, suggesting that distinct mechanisms may be involved in their development and differentiation. The splenic red pulp macrophages present in unmanipulated op/op mice are an M-CSF-independent macrophage population. Although the marginal metallophilic macrophages and marginal zone macrophages are thought to be M-CSF-dependent, their development and differentiation appear to be influenced by locally produced M-CSF or other cytokines.
机译：使用抗小鼠巨噬细胞单克隆抗体和特异于边缘嗜金属巨噬细胞或边缘区巨噬细胞的抗小鼠单克隆抗体的免疫组织化学技术来检测op / op小鼠脾脏中的红浆巨噬细胞，边缘嗜金属巨噬细胞和边缘区巨噬细胞。在突变小鼠中，红髓巨噬细胞减少到正常同窝仔中的约60％，并且没有边缘嗜金属巨噬细胞和边缘带巨噬细胞。施用重组人巨噬细胞集落刺激因子（rhM-CSF）后，红色果肉巨噬细胞数量迅速增加，并在1周后达到正常同窝仔的水平。相反，在施用rhM-CSF后，边缘嗜金属性巨噬细胞以及边缘区巨噬细胞出现缓慢，并且即使在施用12周时，它们的数量也小于正常同窝仔的基线水平的一半。在op / op小鼠的脾脏中，M-CSF给药后出现的边缘嗜金属性巨噬细胞和边缘区巨噬细胞的分布是不规则的。这些脾脏巨噬细胞亚群对rhM-CSF的反应不同，表明它们的发育和分化可能涉及不同的机制。存在于未操作的op / op小鼠中的脾红髓巨噬细胞是不依赖M-CSF的巨噬细胞群体。尽管边缘嗜金属巨噬细胞和边缘区巨噬细胞被认为是M-CSF依赖性的，但它们的发育和分化似乎受到局部产生的M-CSF或其他细胞因子的影响。

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