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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Partial characterization of the proliferative activity for fetal lung epithelial cells produced by silica-exposed alveolar macrophages.
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Partial characterization of the proliferative activity for fetal lung epithelial cells produced by silica-exposed alveolar macrophages.

机译：暴露于二氧化硅的肺泡巨噬细胞产生的胎儿肺上皮细胞增殖活性的部分表征。

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摘要

The proliferation of lung epithelial cells is a prominent feature of lung tissue response following silica-induced lung injury and alveolar macrophages are recognized as a major contributing cell to the lung inflammatory processes. In previous studies, a growth-promoting activity for fetal rat lung epithelial cells was observed in silicotic alveolar fluids and in supernatants from in vitro and in vivo silica-exposed alveolar macrophages. In the present work, the biological and physicochemical properties of the macrophage-derived growth-promoting activity for fetal lung epithelial cells were explored. Four peaks of growth-promoting activity for lung epithelial cells ranging from 32 to 7 kDa were found in both in vitro and in vivo silica-exposed macrophage supernatants. The investigations were coupled with biochemical treatments of the mitogenic peaks and blocking antibodies or antisera were used to specify further the nature of the proliferative activities. Among the established growth factors, alveolar macrophage-derived growth fractions had characteristics consistent with platelet-derived growth factor-, insulin-like growth factor 1-, and fibroblast growth factor-like molecules. The cytokine production following in vitro exposure, which reflects very early events in the pathogenesis of silicosis, was more strongly related to the high-molecular-weight PDGF-like molecules, whereas the cytokine production following in vivo exposure, which reflects later events in the pathogenesis of silicosis, was more influenced by intermediate-molecular-weight FGF- and IGF-like molecules.
机译：二氧化硅诱导的肺损伤后，肺上皮细胞的增殖是肺组织反应的突出特征，并且肺泡巨噬细胞被认为是肺炎性过程的主要贡献细胞。在先前的研究中，在矽肺泡液和体外和体内暴露于二氧化硅的肺泡巨噬细胞的上清液中观察到了对胎儿大鼠肺上皮细胞的生长促进活性。在目前的工作中，探索了巨噬细胞对胎儿肺上皮细胞的促生长活性的生物学和理化性质。在体外和体内暴露于二氧化硅的巨噬细胞上清液中都发现了32至7 kDa的四个肺上皮细胞生长促进活性峰。这些研究与有丝分裂峰的生化处理相结合，并使用阻断抗体或抗血清进一步说明了增殖活性的性质。在确定的生长因子中，肺泡巨噬细胞衍生的生长级分具有与血小板衍生的生长因子，胰岛素样生长因子1-和成纤维细胞生长因子样分子一致的特征。体外暴露后的细胞因子产生反映了矽肺病发病的早期事件，与高分子量PDGF样分子密切相关，而体内暴露后的细胞因子产生反映了矽肺病后期的事件。矽肺的发病机制受中等分子量FGF和IGF样分子的影响更大。

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