An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells

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An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells

机译：一种优化的人肺肿瘤分解方法，可保留免疫细胞的表型和功能

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Carefulpreparationofhumantissuesisthecornerstoneofobtainingaccuratedatainimmunologicstudies.Despitetheessentialimportanceoftissueprocessingintumorimmunologyandclinicalmedicine,currentmethodsoftissuedisaggregationhavenotbeenrigorouslytestedfordatafidelity.Thus,wecriticallyevaluatedthecurrenttechniquesavailableintheliteraturethatareusedtopreparehumanlungtumorsforimmunologicstudies.WediscoveredthattheseapproachesaresuccessfulatdigestingcellularattachmentsandECMs;however,thesemethodsfrequentlyaltertheimmunecellcompositionand/orexpressionofsurfacemolecules.Wethusdevelopedanovelapproachtopreparehumanlungtumorsforimmunologicstudiesbycombininggentlemechanicalmanipulationwithanoptimizedcocktailofenzymesusedatlowdoses.Thisenzymaticdigestioncocktailoptimizedcellyieldandcellviability,retrievedallmajortumor‐associatedcellpopulations,andmaintainedtheexpressionofcell‐surfacemarkersforlineagedefinitionandinvivoeffectorfunctions.Toourknowledge,wepresentthefirstrigorouslytesteddisaggregationmethoddesignedforhumanlungtumors...

机译：Carefulpreparationofhumantissuesisthecornerstoneofobtainingaccuratedatainimmunologicstudies.Despitetheessentialimportanceoftissueprocessingintumorimmunologyandclinicalmedicine，currentmethodsoftissuedisaggregationhavenotbeenrigorouslytestedfordatafidelity.Thus，wecriticallyevaluatedthecurrenttechniquesavailableintheliteraturethatareusedtopreparehumanlungtumorsforimmunologicstudies.WediscoveredthattheseapproachesaresuccessfulatdigestingcellularattachmentsandECMs;然而，thesemethodsfrequentlyaltertheimmunecellcompositionand / orexpressionofsurfacemolecules.Wethusdevelopedanovelapproachtopreparehumanlungtumorsforimmunologicstudiesbycombininggentlemechanicalmanipulationwithanoptimizedcocktailofenzymesusedatlowdoses.Thisenzymaticdigestioncocktailoptimizedcellyieldandcellviability，retrievedallmajortumor-associatedcellpopulations，andmaintainedtheexpressionofcell-surfacemarkersforlineagedefinitionandinvivoeffectorfunctions.Toourknowledge，wepresentthefirstrigorouslytesteddisaggregationmethoddesign edfor人类肺癌...

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《Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society》 |2015年第1期|共9页
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1. Technical Advance: An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells [J] . Quatromoni Jon G., Singhal Sunil, Bhojnagarwala Pratik, Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society . 2015,第1期

机译：技术进步：一种优化的人肺肿瘤分解方法，可保留免疫细胞的表型和功能
2. Spherical cell shape of FLC-4 cell, a human hepatoma cell, enhances hepatocyte-specific function and suppresses tumor phenotype through the integration of mRNA?￠????microRNA interaction Spherical cell shape of FLC-4 cell, a human hepatoma cell, enhances hepatocyte-specific function and suppresses tumor phenotype through the integration of mRNA?￠????microRNA interaction Spherical cell shape of FLC-4 cell, a human hepatoma cell, enhances hepatocyte-specific function and suppresses tumor phenotype through the integration of mRNA?￠????microRNA interaction [J] . Misaki Ando, Hiroaki Oda, Thomas Laurent, Biology Open . 2012,第10期

机译：人肝癌细胞FLC-4细胞的球形细胞形状通过整合mRNA micromicroRNA相互作用增强肝细胞特异性功能并抑制肿瘤表型，人肝癌细胞FLC-4细胞的球形细胞形状，通过整合mRNA？microRNA相互作用增强肝细胞特异性功能并抑制肿瘤表型。人肝癌细胞FLC-4细胞的球形细胞形状，通过整合mRNA增强肝细胞特异性功能并抑制肿瘤表型microRNA相互作用
3. Natural killer cells in the human lung tumor microenvironment display immune inhibitory functions [J] . Jules Russick, Pierre-Emmanuel Joubert, Mélanie Gillard-Bocquet, Journal for ImmunoTherapy of Cancer . 2020,第2期

机译：人肺肿瘤微环境的天然杀伤细胞显示免疫抑制功能
4. Antitumor Effect and Immune Regulation of The Pingyan Capsule on Lewis Lung Cancer Cells in Vivo [C] . Na LI, Yan LI, Tian-yu LIU International Conference on Biomedical and Biological Engineering . 2017

机译：抗肿瘤胶囊在体内Lewis肺癌细胞上的抗肿瘤效应和免疫调节
5. Gene expression analysis of human non -small cell lung cancer subtypes, adenocarcinoma and squamous cell carcinoma, and mouse lung epithelial cell lines: Implications in lung tumorigenesis [D] . Silvers, Amy Lynn. 2002

机译：人类非小细胞肺癌亚型，腺癌和鳞状细胞癌以及小鼠肺上皮细胞系的基因表达分析：在肺肿瘤发生中的意义
6. An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells [O] . Jon G. Quatromoni, Sunil Singhal, Pratik Bhojnagarwala, -1

机译：一种优化的人肺肿瘤分解方法可保留免疫细胞的表型和功能
7. An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells [O] . Jon G. Quatromoni, Sunil Singhal, Pratik Bhojnagarwala, 2014

机译：人肺肿瘤的优化分解方法，其保留免疫细胞的表型和功能

An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells

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