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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >The murine IL-8 homologues KC, MIP-2, and LIX are found in endothelial cytoplasmic granules but not in Weibel-Palade bodies
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The murine IL-8 homologues KC, MIP-2, and LIX are found in endothelial cytoplasmic granules but not in Weibel-Palade bodies

机译：鼠IL-8同源物KC，MIP-2和LIX在内皮细胞质颗粒中发现，但在Weibel-Palade体中未发现

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Rapid translocation of P-selectin from WPB to the surface of endothelial cells is crucial for early neutrophil recruitment to acute inflammatory lesions. Likewise, the chemokine CXCL8/IL-8 is sorted to WPB in human endothelial cells, but little is known about its functional importance in lack of a suitable animal model. Here, we explored the distribution of the functional IL-8 homologues CXCL1/KC, CXCL2/MIP-2, and CXCL5-6/LIX in resting and inflamed murine vessels by confocal microscopy and paired immunostaining with markers of WPB, discovering that these chemokines did not localize to WPB but displayed a granular pattern in a subset of vessels in healthy skin compatible with sorting to the type 2 endothelial compartment for regulated secretion. Moreover, all chemokines colocalized with VWF and P-selectin in platelets, suggesting that their storage in platelet ?±-granules might represent an alternative source of rapidly available, neutrophil-recruiting chemokines. In conclusion, WPB appear not to be involved in regulated secretion of chemokines in the mouse, and instead, the possible existence of type 2 granules and the role of platelets in rapid leukocyte adhesion deserve further attention.
机译：P-选择蛋白从WPB到内皮细胞表面的快速转运对于早期嗜中性粒细胞募集到急性炎症病变至关重要。同样，趋化因子CXCL8 / IL-8在人内皮细胞中被分类为WPB，但由于缺乏合适的动物模型而对其功能的重要性知之甚少。在这里，我们通过共聚焦显微镜研究了功能性IL-8同源物CXCL1 / KC，CXCL2 / MIP-2和CXCL5-6 / LIX在静止和发炎的鼠类血管中的分布，并与WPB标记配对免疫染色，发现这些趋化因子未定位于WPB，但在健康皮肤的一部分血管中显示了颗粒状图案，与分选到2型内皮区隔以调节分泌有关。此外，所有趋化因子均与血小板中的VWF和P-选择素共定位，这表明它们在血小板α-颗粒中的储存可能代表了可快速获得的，嗜中性白细胞趋化因子的替代来源。总之，WPB似乎不参与小鼠趋化因子的调节分泌，相反，可能存在2型颗粒以及血小板在快速白细胞粘附中的作用值得进一步关注。

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