Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry

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Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry

机译：用多色流式细胞术表征恒河猴的血液，骨髓和次级淋巴器官中的B和浆细胞

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Bcells,asanimportantpartofthehumoralimmuneresponse,aregeneratedintheBM,migratetosecondarylymphoidorgans,anduponactivation,differentiateintoantibody‐producingmemoryBcellsorplasmacells.Despitethepivotalrolesthattheyplayindifferentdiseases,acomprehensivecharacterizationinhealthyrhesusmacaques,whichserveasvaluablemodelsforavarietyofhumandiseases,isstillmissing.Withtheuseofmultiparameterflowcytometry,weanalyzedBcellsinBMcollectedfromtwolocations,i.e.,theiliaccrest(BMca)andthefemur(BMfem),PB,aswellassecondarylymphoidorgansofhealthyrhesusmacaques.WeassessedthefrequenciesofimmatureandmatureBcells,aswellasCD19+CD20–CD38+/++CD138+/++plasmablasts/plasmacells.Furthermore,wefoundsite‐specificdifferencesintheexpressionofmarkersforBcellactivationandproliferation,chemokinereceptorsandIgs,aswellasthedistributionofmemoryBcellsubpopulations.AssecondarylymphoidorgansharborthehighestfrequenciesofnaiveBcells,expressionofCD80,CD95,andKi67waslowercomparedwithBcellsintheperipheryandBM,whereasexpressionofIgD,CXCR4(CD184),andCCR7(CD197)washigher.Interestingly,BMcadifferedfromBMfemregardingfrequenciesofBcells,theirexpressionofCD80andCXCR4,Tcells,andplasmacells.Insummary,thesedataidentifybaselinevaluesfortheabove‐mentionedparametersandprovidethefoundationforfuturestudiesonBandplasmacellsindifferentdiseases...

机译：B细胞，asanimportantpartofthehumoralimmuneresponse，aregeneratedintheBM，migratetosecondarylymphoidorgans，anduponactivation，differentiateintoantibody-producingmemoryBcellsorplasmacells.Despitethepivotalrolesthattheyplayindifferentdiseases，acomprehensivecharacterizationinhealthyrhesusmacaques，whichserveasvaluablemodelsforavarietyofhumandiseases，isstillmissing.Withtheuseofmultiparameterflowcytometry，weanalyzedBcellsinBMcollectedfromtwolocations，即theiliaccrest（BMCA）andthefemur（BMfem），PB，aswellassecondarylymphoidorgansofhealthyrhesusmacaques.WeassessedthefrequenciesofimmatureandmatureBcells，aswellasCD19 + CD20-CD38 + / + + CD138 + / ++成浆细胞/浆细胞。此外，我们发现了针对B细胞活化和增殖的标志物，趋化因子受体和Igs的位点特异性差异，以及记忆B细胞亚群的分布，X线，X线，K线，低CD率，CDB的最高频率，CDB的低频率，CDC的低频率，CDB的最高频率，95有趣的是，对于B细胞的频率，表达CD80和CXCR4，T细胞和浆细胞的表达，BM与BM的频率不同，因此有趣的是，这些数据确定了上述参数的基线值，并为该参数提供了基础。

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《Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society》 |2015年第1期|共12页
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1. Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry [J] . Neumann Berit, Klippert Antonina, Raue Katharina, Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society . 2015,第1期

机译：用多色流式细胞术表征恒河猴的血液，骨髓和次级淋巴器官中的B和浆细胞
2. Quantification and immunophenotypic characterization of bone marrow and umbilical cord blood mesenchymal stem cells by multicolor flow cytometry. [J] . Martins AA, Paiva A, Morgado JM Transplantation Proceedings . 2009,第3期

机译：通过多色流式细胞术对骨髓和脐带血间充质干细胞进行定量和免疫表型鉴定。
3. Cutting edge: IL-21 derived from human follicular helper T cells acts as a survival factor for secondary lymphoid organ, but not for bone marrow, plasma cells [J] . Rodríguez-BayonaB., Ramos-AmayaA., BernalJ., The Journal of Immunology: Official Journal of the American Association of Immunologists . 2012,第4期

机译：尖端技术：源自人卵泡辅助性T细胞的IL-21可作为继发性淋巴器官的生存因子，但对骨髓，浆细胞却不起作用
4. Fluorescent Activated Cell Sorting for the Characterization of Primary Rabbit Bone Marrow Fat Cell, Bone Marrow Stromal Cell, and Osteoblastic Cell Populations [C] . Michelle D. Kofron, Duron Lee, Cato T. Laurencin Society for Biomaterials Transaction of the 31st Annual Meeting vol.XXIX pt.2 . 2006

机译：荧光激活细胞分选以表征原代兔骨髓脂肪细胞，骨髓基质细胞和成骨细胞
5. Rapidly self-renewing stem cells in human bone marrow: The identification, characterization and regulation of a unique bone marrow stromal cell sub-population. [D] . Colter, David Christopher. 2001

机译：人类骨髓中快速自我更新的干细胞：独特的骨髓基质细胞亚群的鉴定，表征和调节。
6. Development of an unbiased semi-automated approach for classifying plasma cell immunophenotype following multicolor flow cytometry of bone marrow aspirates [O] . Steven R. Post, Ginell R. Post, Dejan Nikolic, -1

机译：骨髓穿刺物多色流式细胞术后无偏见半自动化方法对浆细胞免疫表型进行分类的开发
7. Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry [O] . JG Bender, KL Unverzagt, DE Walker, 1991

机译：用多色流式细胞术鉴定和对正常外周血和骨髓群的鉴定及其群体
8. Differences in Lifespan and Rate of Turnover Between Phytohemagglutinin Responsive Cells of the Bone Marrow and of Peripheral Lymphoid Organs [R] . Press, O. W., Rosse, C. 1977

机译：植物血凝素响应细胞骨髓和外周淋巴器官的寿命和转换率差异

Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry

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