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首页> 外文期刊>Journal of Agricultural Science >Biofertigation of Forage With Effluents of Green Line of a Cattle Slaughterhouse: Microbial Diversity and Leaf Dry Mass Productivity
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Biofertigation of Forage With Effluents of Green Line of a Cattle Slaughterhouse: Microbial Diversity and Leaf Dry Mass Productivity

机译:牛屠宰场绿线废料对草料的生物肥力:微生物多样性和叶片干物质生产率

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The wastewater has been an environmental problem, but your used as fertilizers could reduce or eliminate the application of commercial fertilizers in soil. Arbuscular mycorrhizal fungi (AMF) and nitrogen fixing bacteria (NFB) are a good parameter to analyze the impacts of this fertigationon soil. We aimed to evaluate the distribution and diversity of AMF and NFB before and after applications of wastewater or manure from green line of a cattle slaughterhouse in the irrigation of B. brizantha cv Marandu in Cerrado soil and leaf biomass productivity. The experimental design was performed in completely randomized blocks with ten biofertigation managements. The seeds of the forage were distributed in grooves with spacing of 5 cm. This seeds were covered with a soil layer. NFB and AMF diversity was performed by denaturing gradient gel electrophoresis (DGGE). The leaf biomass productivity in the biofertigation managements was higher than in the managements without the use wastewater/manure. After biofertigation managements, changes in the DGGE profile of the NFB and AMF communities were observed. These changes may be due to the difference in the sample collection period and in the soil humidification. Thus, these DGGE profiles was a good parameter to diagnose the efficacy of wastewater/manure as an alternative biotechnological irrigation.
机译:废水一直是一个环境问题,但是您用作肥料可以减少或消除在土壤中使用商业肥料。丛枝菌根真菌(AMF)和固氮菌(NFB)是分析这种施肥对土壤影响的良好参数。我们的目的是评估在塞拉多土壤和叶片生物量生产力中,B。brizantha cv Marandu灌溉中牛屠宰场绿线施用的废水或粪肥施用前后的AMF和NFB的分布和多样性。实验设计是在十种生物施肥管理的完全随机分组中进行的。饲草的种子分布在间距为5 cm的凹槽中。该种子被土壤层覆盖。 NFB和AMF多样性是通过变性梯度凝胶电泳(DGGE)进行的。生物施肥管理中的叶片生物量生产力要高于不使用废水/粪肥的管理中。经过生物施肥管理后,观察到NFB和AMF群落的DGGE谱发生了变化。这些变化可能是由于样品采集时间和土壤湿度的不同所致。因此,这些DGGE曲线是诊断废水/粪便作为替代性生物技术灌溉的功效的良好参数。

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