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首页> 外文期刊>Journal of Medical Biochemistry >Association of Serum Paraoxonase 1 Activities, Polymorphisms and Oxidative Stress in Breast Cancer Patients with Type 2 Diabetes Mellitus
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Association of Serum Paraoxonase 1 Activities, Polymorphisms and Oxidative Stress in Breast Cancer Patients with Type 2 Diabetes Mellitus

机译：乳腺癌2型糖尿病患者血清对氧磷酶1活性，多态性与氧化应激的关系

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Summary Background The aim of the study was to investigate the association of paraoxonase 1 (PON1) polymorphism, PON1/arylesterase (ARE) activity and oxidative stress index (OSI) in breast cancer (BC) patients with type 2 diabetes (DM). Methods Our study group consisted of 30 healthy women (HV group) and 66 female BC patients. The BC patients were divided into two groups: those with (n=37) and without DM (n=29) (BDM and NBDM group). Genotyping of PON1 Q192R and L55M polymorphisms were done by polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) method. Serum PON1/ARE enzyme activities, total oxidant status (TOS) and total antioxidant status (TAS) were analysed by spectrophotometric method. The ratio of TOS to TAS was accepted as the oxidative stress index (OSI). Results PON1 Q192R genotype frequency distribution was significantly different in the BDM group compared to the NBDM group (p=0.021). When alleles distribution was examined, R and L alleles were significantly lower, Q and M alleles were significantly higher in the BDM group than in the NBDM group (p&0.001). TOS and OSI were statistically higher in BC patients than HV group (p&0.001). Conclusions Our results suggest that PON1 gene Q and M alleles may be the risk factors predisposing formation of BC due to increased oxidant damage seen in DM. However, these statements require further confirmation with screening PON1 polymorphism in a greater number of patients with DM, and also wide range follow-up studies are necessary for the same purpose.
机译：发明背景研究的目的是研究对2型糖尿病（DM）乳腺癌（BC）患者的对氧磷酶1（PON1）多态性，PON1 /芳基酯酶（ARE）活性和氧化应激指数（OSI）的相关性。方法我们的研究组由30名健康女性（HV组）和66名女性BC患者组成。 BC患者分为两组：有（n = 37）和无DM（n = 29）（BDM和NBDM组）。 PON1 Q192R和L55M多态性的基因分型通过聚合酶链反应（PCR）–限制性片段长度多态性（RFLP）方法完成。用分光光度法分析了血清PON1 / ARE的酶活性，总氧化剂状态（TOS）和总抗氧化剂状态（TAS）。 TOS与TAS的比例被认为是氧化应激指数（OSI）。结果BDM组的PON1 Q192R基因型频率分布与NBDM组相比有显着差异（p = 0.021）。当检查等位基因分布时，BDM组中的R和L等位基因显着较低，Q和M等位基因显着高于NBDM组（p ＜0.001）。 BC患者的TOS和OSI在统计学上高于HV组（p ＜0.001）。结论我们的结果表明PON1基因Q和M等位基因可能是由于DM中氧化损伤增加而诱发BC形成的危险因素。然而，这些说法需要在更多的DM患者中筛查PON1多态性来进一步证实，并且出于相同目的还需要进行广泛的随访研究。

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