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首页> 外文期刊>Journal of medical bacteriology. >Determination of Antimicrobial Resistance Pattern and Detection of blaTEM Gene among Clinical Isolates of Escherichia coli
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Determination of Antimicrobial Resistance Pattern and Detection of blaTEM Gene among Clinical Isolates of Escherichia coli

机译：大肠杆菌临床分离株中抗菌素耐药性模式的确定和blaTEM基因的检测

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Background: Unfortunately, antibiotic resistance has become an increasingly critical problem in many countries like Iran. Since there are very few published data on antibiotics resistance in Alborz province, the aim of this study was to survey the pattern of antimicrobial resistance and prevalence detection of TEM-type beta-lactamases among clinical isolates of Escherichia coli using universal primers.?Methods: The study was performed on 83 clinical Escherichia coli strains collected from hospitals and clinical laboratories. Antimicrobial susceptibility was performed using Kirby-Bauer disk diffusion method against common antibiotics. Isolates were also screened for the production of extended spectrum beta-lactamases (ESBLs) by double disk synergy test (DDST). Positive isolates were evaluated by PCR analysis for the TEM family of ESBLs genes.?Results: Isolates showed the highest resistance to amoxicillin (83%), whereas nitrofurantoin was the most effective drug, with only 8.4% resistance. The frequency of multi drug resistance (MDR) to more than 5 antibiotics was 79.5% (66 strains). ESBL screening of E. coli strains by DDST showed that out of 83 strains, 33 isolates were ESBL positive. Based on the PCR results 61 percent of phenotypic ESBL positive E. coli isolates possessed a single gene encoding a TEM type ESBL.Conclusion: As the results of this study indicate, multidrug resistance is an increasing therapeutic concern and treatment requires further attention to the results of susceptibility tests. As antibiotic options in the treatment of ESBL-producing organisms are extremely limited, molecular screening by laboratories is suggested to reduce the risk of therapeutic defeat.
机译：背景：不幸的是，在许多国家（如伊朗），抗生素耐药性已成为日益严重的问题。由于在Alborz省很少有关于抗生素耐药性的公开数据，因此本研究的目的是调查使用通用引物对大肠杆菌临床分离株中的抗生素耐药性模式和TEM型β-内酰胺酶的流行程度进行检测。该研究是对从医院和临床实验室收集的83株临床大肠杆菌菌株进行的。使用Kirby-Bauer纸片扩散法对常用抗生素进行抗菌药敏试验。还通过双盘协同试验（DDST）筛选了分离物以生产超广谱β-内酰胺酶（ESBL）。结果：分离株对阿莫西林的耐药性最高（83％），而呋喃妥因是最有效的药物，耐药率仅为8.4％。对5种以上抗生素的多重耐药性（MDR）频率为79.5％（66个菌株）。通过DDST对大肠杆菌菌株进行ESBL筛选显示，在83株菌株中，有33株分离株为ESBL阳性。根据PCR结果，表型ESBL阳性大肠杆菌分离株中有61％拥有一个编码TEM型ESBL的单一基因。结论：如本研究结果所示，多药耐药性正在日益引起人们的治疗关注，治疗需要进一步关注结果药敏试验。由于治疗ESBL产生生物的抗生素选择极为有限，因此建议实验室进行分子筛查以降低治疗失败的风险。

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