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首页> 外文期刊>Journal of medical bacteriology. >Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium
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Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium

机译：含有胶体纳米银的琼脂水凝胶对金黄色葡萄球菌和鼠伤寒沙门氏菌的生物膜去除和抗菌活性。

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Background: Antibacterial and biofilm removal effects of agar hydrogel incorporating silver nanoparticles (SNP) at various concentrations were studied against Staphylococcus aureus and Salmonella typhimurium in vitro.Methods: The minimum inhibitory concentrations (MIC) of SNP was determined by agar dilution method. Then, hydrogels were prepared by mixing of 0.5% w/v agar and SNP (1/2 MIC, MIC, and 2 MIC) and their inhibitory efficacies against planktonic and biofilm forms of bacteria were measured using agar spot and microtiter test, respectively.Results: The MIC value was 125 μg/ mL for both bacteria. All SNP hydrogels represented antibacterial activity against Staphylococcus aureus and S. typhimurium on agar culture, which was significant compared to control group (silver sulfadiazine cream). The developed biofilm of S. aureus and S. typhimurium were strongly (85% reduction) and modernly affected (60% reduction) by SNP hydrogels during 15 min contact time, respectively. A dose-dependent biofilm reduction was not demonstrated when different SNP concentrations were tested. Moreover, the results from this study confirmed the moderate sanitizing ability of SNP loaded hydrogel against planktonic forms of both bacteria, which SNP (2MIC) hydrogel decreased only 2.3 log10 CFU/ mL in a primary population of S. typhimurium during 15 min exposure time.Conclusion: We recommended SNP incorporated agar hydrogel as an effective biofilm removal sanitizer.
机译：背景：研究了不同浓度的掺入银纳米颗粒（SNP）的琼脂水凝胶对金黄色葡萄球菌和鼠伤寒沙门氏菌的体外抗菌和生物膜去除作用。方法：通过琼脂稀释法确定SNP的最低抑菌浓度（MIC）。然后，将0.5％w / v琼脂和SNP（1/2 MIC，MIC和2 MIC）混合制备水凝胶，并分别通过琼脂斑点法和微量滴定法测定其对浮游细菌和生物膜形式细菌的抑制作用。结果：两种细菌的​​MIC值为125μg/ mL。所有SNP水凝胶在琼脂培养上均对金黄色葡萄球菌和鼠伤寒沙门氏菌具有抗菌活性，与对照组（磺胺嘧啶银乳膏）相比具有显着意义。在15分钟的接触时间内，SNP水凝胶对金黄色葡萄球菌和鼠伤寒沙门氏菌的生物膜形成了强烈的影响（减少了85％），现代受到了影响（减少了60％）。当测试不同的SNP浓度时，未证明剂量依赖性生物膜减少。此外，这项研究的结果证实了负载SNP的水凝胶对两种细菌的​​浮游形式具有中等的杀菌能力，在15分钟的暴露时间内，SNP（2MIC）水凝胶在鼠伤寒沙门氏菌的主要种群中仅降低了2.3 log10 CFU / mL。结论：我们建议将SNP掺入琼脂水凝胶作为一种有效的生物膜去除消毒剂。

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