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首页> 外文期刊>Journal of medical bacteriology. >Prevalence and Evaluation of Toxin Genes among Uropathogenic Escherichia coli Clinical Isolates by Duplex PCR
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Prevalence and Evaluation of Toxin Genes among Uropathogenic Escherichia coli Clinical Isolates by Duplex PCR

机译：双重PCR在尿毒症大肠杆菌临床分离株中毒素基因的流行与评价。

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Background: One of the most common infections in human is urinary tract infection (UTI) and Uropathogenic Escherichia coli is one of its major causative agents. UTI is extremely common among young women. Children under age 5 are also highly at risk. Considering the prevalence of this disease, it is necessary to design an appropriate diagnostic method for its effective diagnosis. The aim of present study was to identify the prevalence of two virulence genes (sat and vat) among Uropathogenic E. coli isolates.Methods: Urine samples were taken from 350 patients with urinary tract infection. The samples were cultured on EMB agar and Blood agar. The suspected E. coli colonies were isolated and confirmed by biochemical tests. The genomic DNA was extracted from 297 isolated E. coli and target genes were amplified by PCR. The amplicons were sequenced and analyzed with ClustalW software. Moreover, data analysis was performed by using SPSS software. Subsequently, Duplex PCR was optimized for simultaneous detection of two genes.Results: The prevalence of sat and vat genes were 75 (n: 225) and 36 (n: 106)percent, respectively. In addition, less than 4% (n: 11) of clinical isolates comprised two genes.Conclusion: According to the conducted research, molecular identification of Uropathogenic E .coli strains according to detection of sat gene is potentially an appropriate method and could be noted for diagnosis.
机译：背景：人类中最常见的感染之一是尿路感染（UTI），而致病性大肠杆菌是其主要病原体之一。尿路感染在年轻女性中极为普遍。 5岁以下的儿童也处于高度危险之中。考虑到这种疾病的流行，有必要设计一种有效的诊断方法。本研究的目的是确定在致病性大肠杆菌分离物中两个毒力基因（sat和vat）的流行程度。方法：从350例尿路感染患者中采集尿液样本。将样品在EMB琼脂和血琼脂上培养。分离出可疑的大肠杆菌菌落，并通过生化测试确认。从297株分离的大肠杆菌中提取基因组DNA，并通过PCR扩增靶基因。对扩增子进行测序并用ClustalW软件进行分析。此外，通过使用SPSS软件进行数据分析。随后，对Duplex PCR进行了优化，以同时检测两个基因。结果：sat和vat基因的患病率分别为75（n：225）和36（n：106）％。此外，不到4％（n：11）的临床分离株包含两个基因。结论：根据进行的研究，根据sat基因检测对致病性大肠杆菌进行分子鉴定可能是一种合适的方法，值得一提。用于诊断。

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