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首页> 外文期刊>Journal of medical bacteriology. >An In Vitro Assessment of Antimicrobial and Cytotoxic Effects of Nanosilver
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An In Vitro Assessment of Antimicrobial and Cytotoxic Effects of Nanosilver

机译：纳米银的抗菌和细胞毒性作用的体外评估

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Background: The antimicrobial activity of silver nanoparticles has been investigated in medical fields in recent years, but there are few studies regarding its effect on oral microorganisms. The aim of the present study was to evaluate the in vitro antimicrobial and toxicity properties of nanosilver against two dental plaque microorganisms and Human Gingival Fibroblast (HGF) cell line.Methods: Antibacterial effects of nanosilver colloidal solution were determined by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) using microdilution method. Standard strains of Streptococcus sanguis and Actinomyces viscosus were used. For toxicity assessment,?MTT?and LDH tests were performed under con- trolled conditions. Different concentrations of nanosilver were prepared and their toxic effects on HGF were determined after 24, 48 and 72 hours.Results: The MIC of nanosilver solution for S. sanguis and A. viscosus were 16 and 4 μ g/ml, respectively. The MBC of nanosilver was 64 μ g/ml for S. sanguis and 16 μ g/ml for A. viscosus. MTT results showed that after 24 hours the concentrations of ≥ 0.5 μ g/ml of nanosilver solution affected cell viability when compared with control group. After 48 and 72 hours only the concentration of ≥ 5 μ g/ml showed significant effect on cultured cell viability. LDH release test demonstrated toxic effect only after 48, 72 hours by 20 and 50 μ g/ml of nanosilver.Conclusion: The results demonstrated that beside its antibacterial activity against S. sanguis and A. viscosus, nanosilver mediated a concentration and time dependent cytotoxicity on HGF.
机译：背景：近年来，在医学领域已经研究了银纳米颗粒的抗菌活性，但是关于其对口腔微生物的作用的研究很少。本研究的目的是评估纳米银对两种牙菌斑微生物和人牙龈成纤维细胞（HGF）细胞系的体外抗菌和毒性特性。微量稀释法测定最低杀菌浓度（MBC）。使用标准链球菌和粘性放线菌的菌株。为了进行毒性评估，在受控条件下进行了“ MTT”和LDH测试。制备了不同浓度的纳米银，并在24、48和72小时后测定了它们对HGF的毒性作用。结果：纳米银溶液对桑氏曲霉和粘链曲霉的MIC分别为16和4μg/ ml。血红链霉菌的纳米银的MBC为64μg / ml，粘链曲霉的MBC为16μg / ml。 MTT结果显示，与对照组相比，24小时后浓度≥0.5μg / ml的纳米银溶液会影响细胞活力。在48和72小时后，仅≥5μg / ml的浓度对培养的细胞活力显示出显着影响。 LDH释放试验仅在48、72小时后分别以20和50μg / ml的纳米银表现出毒性作用。结论：结果表明，纳米银除了具有对血球链球菌和粘液曲霉的抗菌活性外，还介导了浓度和时间依赖性的细胞毒性。在HGF上。

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