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首页> 外文期刊>Journal of Medical Sciences >Expression of Micro RNA-21 and 29c in Blood Plasma of Patients with Nasopharyngeal Carcinoma
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Expression of Micro RNA-21 and 29c in Blood Plasma of Patients with Nasopharyngeal Carcinoma

机译：鼻咽癌患者血浆微RNA-21和29c的表达

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MicroRNA (miRNA) are small non-coding RNA molecules that work in post-transcription and can cause degradation and cell damage through the targeted gene. Damage to these cells influences the process of carcinogenesis. One of them is in nasopharyngeal carcinoma (NPC). Among families of miRNA that play a role in the pathogenesis of NPC that miRNA-21, miRNA-29c and which is closely associated with the progression, invasion and tumor metastasis. The purpose of this study was to determine the expression of miRNA-21 and miRNA-29c. Knowledge of these miRNA could help in the development of targeted therapy in carcinoma, as a biomarker diagnostic and clinical predictive and would be useful in therapeutic decision making. The research design was observational analytic study carried out in Wahidin Sudirohusodo Makassar, January-June, 2017. The collected blood plasma was isolated and then synthesized to cDNA and calculated quantitatively with qRT-PCR and analyzed using CFX manager 96. There were 52 samples consisting of 27 plasma NPC and 25 controls. From the plasma detected miRNA-21 and miRNA-29c. Expression of miRNA-21 increased 4.47795 fold compared with controls (p<0.05)) but its expression can not describe the progression of the tumor at a later stadium (p<0.05). In contrast, the expression of miRNA-29c decreased -119.317 fold compared with control, its regulation at a later stadium did not change significantly. Both miRNA-21 and miRNA-29c in blood plasma can use for NPC detection but a single microRNA examination is less able to provide an overview of progressivity.
机译：MicroRNA（miRNA）是小的非编码RNA分子，可在转录后起作用，并可通过目标基因引起降解和细胞损伤。这些细胞的损伤影响癌变过程。其中之一是鼻咽癌（NPC）。在miRNA的发病机理中起作用的miRNA家族中，miRNA-21，miRNA-29c与其进展，侵袭和肿瘤转移密切相关。这项研究的目的是确定miRNA-21和miRNA-29c的表达。这些miRNA的知识可作为生物标志物的诊断和临床预测手段，帮助开发靶向治疗的癌症，并有助于治疗决策。研究设计是在2017年1月至6月于Wahidin Sudirohusodo Makassar进行的观察分析研究。分离收集的血浆，然后合成为cDNA，并通过qRT-PCR进行定量计算，并使用CFX Manager 96进行分析。共有52个样本27个血浆NPC和25个对照。从血浆中检测到miRNA-21和miRNA-29c。与对照相比，miRNA-21的表达增加了4.47795倍（p <0.05）），但其表达不能描述肿瘤在随后的运动场上的进展（p <0.05）。相反，与对照相比，miRNA-29c的表达降低了-119.317倍，其在后期体育场馆的调控没有明显改变。血浆中的miRNA-21和miRNA-29c均可用于NPC检测，但单次microRNA检测不能提供进展的概况。

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