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Fasciola gigantica Excretory/secretory Antigens as Possible Vaccine Candidates

机译：Fasciola gigantica排泄/分泌抗原可能是疫苗的候选者

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A variety of antigens are secreted and excreted by parasites present in the blood, faeces, urine and other fluids of the infected host. These antigens have potential for use in immunodiagnosis and vaccine development. In an attempt to develop a suitable vaccine against F. gigantica infection, two antigens were isolated and purified from excretory/secretory (E/S) products as cysteine protease and fatty acid binding proteins (CP and FABP) of the parasite by immunoaffinity chromatography. Parasitological and immuno-logical parameters were standardized using the sera from experimentally non-infected (group A) infected (group B), immunized with CP (group C), immunized with CP and infected (group D), immunized with FABP (group E) and immunized with FABP and infected (group F). The mean worm burdens and bile egg count after challenge were reduced significantly by 37.7 and 55.5%, respectively in rabbits vaccinated with CP. In contrast, low significant reduction in worm burdens and bile egg count were observed in rabbits immunized with FABP after challenge (23.5 and 35.7%, respectively). All F. gigantica infected rabbits showed an increased Igs and cytokines levels. On the other hand, immunization of rabbits with CP or FABP induced a significant expression of humoral antibodies (IgM, total IgG, IgG1, IgG2 and IgG4) and cytokines (IL-6, IL-10, IL-12 and TNF-α) with higher level in case of CP than FABP. From this study, we can deduce that F. gigantica CP is a relevant candidate for vaccination against fascioliasis, while the level of protection used by FABP may not appear sufficient enough to protect these ruminants against the deleterious effects of Fasciola infection.
机译：感染的宿主血液，粪便，尿液和其他体液中存在的寄生虫会分泌和排泄多种抗原。这些抗原具有用于免疫诊断和疫苗开发的潜力。为了开发一种适合的针对巨巨镰刀菌感染的疫苗，通过免疫亲和色谱法从寄生虫的半胱氨酸蛋白酶和脂肪酸结合蛋白（CP和FABP）中分离并纯化了两种抗原，即排​​泄物/分泌物（E / S）。寄生虫学和免疫学参数使用来自实验未感染（A组）感染（B组），CP免疫（C组），CP免疫和感染（D组），FABP免疫（E组）的血清标准化）并用FABP免疫并感染（F组）。接种CP的兔子在攻击后平均蠕虫负担和胆汁卵数分别显着降低了37.7和55.5％。相比之下，在攻击后用FABP免疫的兔子中蠕虫负担和胆汁卵计数的降低显着（分别为23.5和35.7％）。所有感染巨无花果的兔子均表现出较高的Igs和细胞因子水平。另一方面，用CP或FABP免疫兔子会诱导体液抗体（IgM，总IgG，IgG1，IgG2和IgG4）和细胞因子（IL-6，IL-10，IL-12和TNF-α）的显着表达。 CP的水平高于FABP。从这项研究中，我们可以推断出巨无花果CP是疫苗接种抗筋膜炎的候选药物，而FABP使用的保护水平似乎不足以保护这些反刍动物免受Fasciola感染的有害影响。

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