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Sequence Heterogeneity of TT Virus

机译：TT病毒的序列异质性

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The aim of this study was to detect blood Transmitted Transfusion Virus (TTV) among Hepatocellular Carcinoma (HCC) patients and investigate the genetic diversity of an isolated TTV strain. Fifty blood samples of patients with HCC non-A through non-G hepatitis were collected and 25 blood donors as controls. TTV DNA was detected by the second generation of TTV PCR primers (NG059, NG061, NG062 and NG063) after extraction of DNA from 50 μL serum. TTV DNA was detected in 90% (45/50) of HCC patients and the prevalence in control group was 40% (10/25). To assess genetic heterogeneity of the TTV genome in more detail, a sequence analysis of PCR fragments, amplified from open reading frame 1 (ORF1) was performed. A total of 137 bp PCR fragments were sequenced and compared to sequences derived from the corresponding TTV genome region deposited in Gen Bank. Present sequencing was closely related to TTV-like mini virus, complete genome (Accession NC_002195), at nucleotide number 855 to 1019. The phylogenetic analysis suggested that present strain might be sub-strain or mutation from the parent gene 2195. These observations suggest a high degree of genetic complexity within the TTV population.
机译：这项研究的目的是在肝细胞癌（HCC）患者中检测血液传播的输血病毒（TTV），并研究分离出的TTV株的遗传多样性。收集了50例HCC非A型至非G型肝炎患者的血液样本，并以25名献血者作为对照。从50μL血清中提取DNA后，通过第二代TTV PCR引物（NG059，NG061，NG062和NG063）检测TTV DNA。在90％（45/50）的HCC患者中检测到TTV DNA，对照组的患病率为40％（10/25）。为了更详细地评估TTV基因组的遗传异质性，对从开放阅读框1（ORF1）扩增的PCR片段进行了序列分析。测序了总共137 bp的PCR片段，并将其与来源于Gen Bank中相应的TTV基因组区域的序列进行了比较。目前的测序与TTV样小病毒完整基因组（登录号NC_002195）密切相关，核苷酸编号为855至1019。系统发育分析表明，目前的菌株可能是来自亲本基因2195的亚菌株或突变。 TTV人群中的高度遗传复杂性。

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