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首页> 外文期刊>Journal of Medicinal Plants Research >Quality assessment of Zaoren-an-shen granule by High-performance liquid chromatography (HPLC) fingerprinting and quantitative analysis
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Quality assessment of Zaoren-an-shen granule by High-performance liquid chromatography (HPLC) fingerprinting and quantitative analysis

机译：高效液相色谱指纹图谱和定量分析法评估枣仁安神颗粒的质量

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摘要

To control the quality of Zaoren-anshen granule (ZRG), a simple, reliable and reproducible method of High-Performance Liquid Chromatography equipped with Photodiode Array Detector (HPLC-DAD) was developed for fingerprint analysis and quantitative determination of three major classes of constituent’s namely phenolic acids, flavonoids and lignanoids. In the fingerprint analysis, nineteen peaks were selected as characteristic peaks and the chemical characteristics of three herbs in ZRG were presented in the HPLC chromatographic file. In the quantitative analysis, one flavonoid spinosin, four phenolic acids including danshensu, protocatechuic acid, protocatechuic aldehyde and salvianolic B and four lignanoids including schisandrin, gomisin A, deoxyschizandrin and gomisin N were successfully separated on an UltimateTM XB-C18 column (250 × 4.6 mm I.D., 5.0 μm) at 35°C. The mobile phase was acetonitrile-water (containing 0.03% phosphoric acid) employing a gradient elution at a flow rate of 1.0 mL/ min, and the detection wavelength was set at 280 nm. Regression equations revealed good linear relationship between the peak areas of the analytes and their concentrations (r > 0.9991) and the recoveries were in the range of 95.5~103.7%. The results indicated that the developed assay method could be considered as a suitable quality control method for ZRG.
机译：为了控制Zaoren-anshen颗粒（ZRG）的质量，开发了一种简单，可靠且可重现的配备光电二极管阵列检测器（HPLC-DAD）的高效液相色谱方法，用于指纹分析和定量测定三大类成分即酚酸，类黄酮和木脂素。在指纹分析中，选择了19个峰作为特征峰，并在HPLC色谱文件中给出了ZRG中三种草药的化学特征。在定量分析中，在UltimateTM XB-C18色谱柱上成功分离出了黄酮类多杀菌素，丹参素，原儿茶酸，原儿茶醛和丹酚B的四种酚酸和五味子素，果胶素A，脱氧五味子素和果胶素N在内的四种木脂素（250×4.6 mm ID，5.0μm）在35°C下。流动相为乙腈-水（含0.03％磷酸），采用1.0 mL / min的流速进行梯度洗脱，检测波长设置为280 nm。回归方程表明，分析物的峰面积与浓度之间具有良好的线性关系（r> 0.9991），回收率在95.5〜103.7％之间。结果表明，所开发的测定方法可以被认为是ZRG的一种合适的质量控制方法。

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