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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >MICROORGANISMS ASSOCIATED WITH COMMERCIAL MOTORCYCLE HELMETS IN LAGOS METROPOLIS
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MICROORGANISMS ASSOCIATED WITH COMMERCIAL MOTORCYCLE HELMETS IN LAGOS METROPOLIS

机译：拉各斯大都会商业摩托车头盔中的微生物

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Microorganisms associated with commercial motorcycle helmets were investigated in the commercial city of Lagos, Nigeria. 300 motorcycle helmets were randomly collected from different commercial motor cyclists in two densely populated areas of Lagos: Yaba College of Technology (YABATECH) and Lagos University Teaching Hospital (LUTH) main gates respectively. Two sterile swabs moistened with sterile water were rotated over the inner surface of each helmet and cultured on MacConkey Agar and Nutrient Agar for bacterial growth and Sabouraud Dextrose Agar for fungi growth. The plates for bacteria growth were incubated aerobically at 37 oC for 48 h, while plates for fungi at 28 oC for 2 weeks. Biochemical tests were used to identify bacteria; while, cultural characteristics were used for fungi identification. The microorganisms consistently common to the samples investigated in the two locations were similar and included (with respective frequency of occurrence for both location): Staphylococcus aureus (80%; 7%), Pseudomonas aeruginosa (75%; 12%), Staphylococcus epidermis (60%; 8%), Enterobacter aerogenes (52%; 27%), Escherichia coli (40%; 13%), Bacillus spp (37%; 10%), Aspergillus spp (82%; 7%), Candida spp (55%; 22%), Rhizopus spp (40%; 27%), and Penicilium spp (35%; 12%). The motorcycle helmets collected at YABATECH had higher microbial colonization than LUTH irrespective of the isolates. This trend was similar for bacterial and fungi. Results showed that helmets could serve as vehicles for transmission of pathogens. Good hygiene practice (GHP) and regular cleaning of motor cycle helmets with sterilants is strongly advocated in order to reduce the incidence of microbial transmission and its associated infection.
机译：在尼日利亚拉各斯的商业城市，研究了与商业摩托车头盔相关的微生物。在拉各斯两个人口稠密的地区分别从Yaba技术学院（YABATECH）和拉各斯大学教学医院（LUTH）主闸口的不同商业摩托车手那里随机收集了300个摩托车头盔。将两个用无菌水浸湿的无菌棉签在每个头盔的内表面上旋转，并在MacConkey琼脂和营养琼脂上进行培养以培养细菌，并在Sabouraud葡萄糖琼脂上进行培养以进行真菌培养。将用于细菌生长的平板在37 oC需氧条件下孵育48小时，而将真菌在28 oC孵育2周。生化测试用于鉴定细菌。同时，将文化特征用于真菌鉴定。在这两个地点所调查的样本中一致共有的微生物是相似的，包括（在两个地点都有各自的出现频率）：金黄色葡萄球菌（80％; 7％），铜绿假单胞菌（75％; 12％），表皮葡萄球菌（ 60％; 8％），产气肠杆菌（52％; 27％），大肠杆菌（40％; 13％），芽孢杆菌（37％; 10％），曲霉菌（82％; 7％），念珠菌（ 55％; 22％），根霉菌属（40％; 27％）和青霉菌属（35％; 12％）。无论分离物如何，在YABATECH收集的摩托车头盔的微生物定植率均高于LUTH。细菌和真菌的趋势相似。结果表明头盔可以作为传播病原体的工具。强烈提倡良好的卫生习惯（GHP）和定期用消毒剂清洁摩托车头盔，以减少微生物传播及其相关感染的发生。

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