• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >ISOLATION AND IDENTIFICATION OF A THERMOTOLERANT PLANT GROWTH PROMOTING PSEUDOMONAS PUTIDA PRODUCING TREHALOSE SYNTHASE
【24h】

ISOLATION AND IDENTIFICATION OF A THERMOTOLERANT PLANT GROWTH PROMOTING PSEUDOMONAS PUTIDA PRODUCING TREHALOSE SYNTHASE

机译：促热假单胞菌产生海藻糖合酶的耐热植物生长的分离与鉴定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A thermotolerant plant growth promoting Pseudomonas isolate growing at 40oC producing trehalose synthase (TreS) was isolated from rhizosphere soil under semi arid conditions of India. Trehalose synthase was extracted; purified and enzymatic activity was examined at various temperatures and pH. The optimum temperature and pH was 38oC and pH 7.5 and the activity declined at above or below the optimum pH and temperature. The enzyme was active on maltose and trehalose among saccharides tested. The enzyme had a higher catalytic activity for maltose with a trehalose yield of 72% than for trehalose where 30% yield of maltose was achieved, indicating maltose as preferred substrate. The isolate showed multiple plant growth promoting traits (indole acetic acid (IAA), phosphate solubilization, siderophore and ammonia) both at ambient (28oC) and high temperature (40oC). Based on phenotypic and 16SrRNA analysis the isolate was identified as Pseudomonas putida (Accession No. GU396283).
机译：在印度的半干旱条件下，从根际土壤中分离出一种能促进耐热植物生长的假单胞菌分离株，该分离株在40oC时产生海藻糖合酶（TreS）。提取海藻糖合酶;在各种温度和pH下检查纯化和酶活性。最适温度和最适pH为38℃，最适pH为7.5，在高于或低于最适pH和最适温度时活性降低。该酶对测试的糖中的麦芽糖和海藻糖有活性。该酶对麦芽糖具有更高的催化活性，海藻糖产率为72％，而海藻糖的麦芽糖产率为30％，表明麦芽糖是优选的底物。该分离物在环境温度（28oC）和高温（40oC）下均表现出多种促进植物生长的特性（吲哚乙酸（IAA），磷酸盐增溶，铁载体和氨）。基于表型和16SrRNA分析，分离物被鉴定为恶臭假单胞菌（保藏号GU396283）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号