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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >OPTIMIZATION OF CELLULASE-FREE XYLANASE PRODUCED BY A POTENTIAL THERMOALKALOPHILIC PAENIBACILLUS SP.N1 ISOLATED FROM HOT SPRINGS OF NORTHERN HIMALAYAS IN INDIA
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OPTIMIZATION OF CELLULASE-FREE XYLANASE PRODUCED BY A POTENTIAL THERMOALKALOPHILIC PAENIBACILLUS SP.N1 ISOLATED FROM HOT SPRINGS OF NORTHERN HIMALAYAS IN INDIA

机译：从印度北部喜马拉雅山热泉中分离得到的潜在热碱性嗜碱杆菌SP.N1产生的无纤维素木聚糖酶的优化

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摘要

Hot spring bacteria are found a novel source of highly active xylanase enzyme with significant activity at high temperature. Among bacteria, Paenibacillus sp.N1 isolated from hot water spring of Manikaran, H.P., India showed highest 24.60 IU.ml-1 of cellulase-free xylanase on Reese medium. Growth conditions including medium, incubation time, pH, temperature, inoculum size, aminoacids, carbon sources, nitrogen sources and additives that affect the xylanase production by Paenibacillus sp.N1 were studied sequentially using the classical “change-one factor at a time” method. The optimal cultivation conditions predicated from canonical analysis of this model were achieved by using basal salt medium on 3rd day, pH 9.0, temperature 50oC with inoculum size of 12.5%, phenylalanine as aminoacid, xylose as carbon source, (NH4)2HPO4 as nitrogen source and Tween 20 as detergent added with an approximate yield of 52.30 IU.ml-1 escalating the over level of xylanase production by 113.38%. A rare combination of all characters i.e. thermoalkalophilic nature and high units of cellulase-free xylanase produced from a new Paenibacillus sp.N1 make it of special industrial interest.
机译：发现温泉细菌是高温下具有显着活性的高活性木聚糖酶的新来源。在细菌中，从印度马尼卡兰（Manikaran）的热水泉中分离出的Paenibacillus sp.N1在Reese培养基中显示出最高的24.60 IU.ml-1的无纤维素酶木聚糖酶。采用经典的“一次变一因子”方法，依次研究了培养基，孵育时间，pH，温度，接种量，氨基酸，碳源，氮源和会影响Paenibacillus sp.N1木聚糖酶产生的添加剂的生长条件。 。通过在第3天使用基础盐培养基，pH 9.0，温度50oC，接种量为12.5％，苯丙氨酸作为氨基酸，木糖作为碳源，（NH4）2HPO4作为氮源，通过对该模型的规范分析确定最佳的培养条件。加入作为洗涤剂的吐温20，产率约为52.30 IU.ml-1，使木聚糖酶的生产水平提高了113.38％。所有特征的罕见组合，即热嗜碱性质和由新的Paenibacillus sp.N1产生的高单位无纤维素酶的木聚糖酶，使其具有特殊的工业价值。

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