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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >AMYLOLYTIC ACTIVITY OF KLUYVER-POSITIVE DEBARYOMYCES OCCIDENTALIS CELLS IMMOBILIZED IN FOAMED ALGINATE GEL
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AMYLOLYTIC ACTIVITY OF KLUYVER-POSITIVE DEBARYOMYCES OCCIDENTALIS CELLS IMMOBILIZED IN FOAMED ALGINATE GEL

机译：泡沫藻酸盐凝胶中固定化的Kluyver阳性十进制酵母菌细胞的淀粉水解活性

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摘要

This study investigates the amylolytic activity of Kluyver-positive yeast strain Debaryomyces occidentalis immobilized in foamed alginate gels. Encapsulation was performed through the traditional process of droplet formation from a foamed alginate solution. The beads were coated with a layer of 3% alginate. Amylolytic enzyme activities were determined in the presence of different carbon sources – glucose, maltose, starch or dextrin – in both complex and minimal culture media. The yeast was capable of producing inducible alpha-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The highest level of cell proliferation was observed in the complex medium with glucose. Immobilized cells showed the highest amylolytic activities in culture media with maltose. Both alpha-amylase and glucoamylase activities were higher in minimal media than in complex media.
机译：本研究调查了固定在泡沫藻酸盐凝胶中的Kluyver阳性酵母菌株Debaryomyces occidentalis的淀粉分解活性。通过从泡沫藻酸盐溶液形成液滴的传统方法进行封装。珠被3％藻酸盐涂层。在复杂和基本培养基中，在不同碳源（葡萄糖，麦芽糖，淀粉或糊精）存在下测定淀粉分解酶的活性。酵母能够产生可诱导的α-淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。在具有葡萄糖的复合培养基中观察到最高水平的细胞增殖。固定化细胞在含麦芽糖的培养基中显示出最高的淀粉分解活性。在基本培养基中，α-淀粉酶和葡糖淀粉酶的活性均高于复杂培养基。

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